{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T05:02:27Z","timestamp":1789534947851,"version":"build-2803163510"},"reference-count":71,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Angew Chem Int Ed"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>N<\/jats:italic>\n                    \u2010 and\n                    <jats:italic>O<\/jats:italic>\n                    \u2010glycosylations are essential post\u2010translational modifications involved in numerous physiological and pathological processes. Sulfated\n                    <jats:italic>N<\/jats:italic>\n                    \u2010 and\n                    <jats:italic>O<\/jats:italic>\n                    \u2010glycans, commonly found on biologically important glycoproteins, serve as critical mediators of molecular recognition. However, their precise structure\u2013function relationships remain poorly understood, primarily due to the limited accessibility of structurally well\u2010defined glycans bearing branch\u2010specific sulfation patterns. Herein, we report a versatile chemoenzymatic strategy that integrates chemical synthesis of editable glycan scaffolds with enzyme\u2010driven diversification, enabling the efficient construction of a diverse library of branch\u2010specifically sulfated\n                    <jats:italic>N<\/jats:italic>\n                    \u2010 and\n                    <jats:italic>O<\/jats:italic>\n                    \u2010glycans, along with their nonsulfated counterparts. Central to the strategy is the streamlined chemical synthesis of editable\n                    <jats:italic>N<\/jats:italic>\n                    \u2010 and\n                    <jats:italic>O<\/jats:italic>\n                    \u2010glycan precursors bearing two orthogonally protected glucosamine termini, allowing branch\u2010selective sulfation through flexible protecting\u2010group manipulation. Subsequent modular enzymatic extension readily generates a panel of sulfated glycans with sialylation and fucosylation patterns. These diverse structures enable comprehensive profiling of their interactions with immune\u2010associated lectins and viral proteins using microarray technology, uncovering unique binding specificities modulated by branch\u2010specific sulfation patterns and core glycan architectures. This work establishes a powerful synthetic approach to previously inaccessible complex sulfated\n                    <jats:italic>N<\/jats:italic>\n                    \u2010 and\n                    <jats:italic>O<\/jats:italic>\n                    \u2010glycans, providing critical tools to advance glycobiology and biomedical applications.\n                  <\/jats:p>","DOI":"10.1002\/anie.2218878","type":"journal-article","created":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T04:10:38Z","timestamp":1789531838000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Chemoenzymatic Assembly of Branch\u2010Selective Sulfated\n                    <i>N<\/i>\n                    \u2010 and\n                    <i>O<\/i>\n                    \u2010Glycans to Decode Glycan\u2010Binding Protein Recognition"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9383-2070","authenticated-orcid":false,"given":"Zhuojia","family":"Xu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Chemical Biology Shanghai Institute of Materia Medica Chinese Academy of Sciences  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianhui","family":"Hao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Chemical Biology Shanghai Institute of Materia Medica Chinese Academy of Sciences  Shanghai China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengzhuo","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica Nanjing University of Chinese Medicine  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica Nanjing University of Chinese Medicine  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Meng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Chemical Biology Shanghai Institute of Materia Medica Chinese Academy of Sciences  Shanghai China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-5047-7397","authenticated-orcid":false,"given":"Jun","family":"Lan","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences Hunan University  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3600-1828","authenticated-orcid":false,"given":"Tiehai","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Chemical Biology Shanghai Institute of Materia Medica Chinese Academy of Sciences  Shanghai China"},{"name":"University of Chinese Academy of Sciences  Beijing China"},{"name":"School of Chinese Materia Medica Nanjing University of Chinese Medicine  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,15]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3383"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.04.012"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2010.11.004"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejcb.2021.151186"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41581\u2010019\u20100129\u20104"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592\u2010022\u201001451\u20100"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA120.015305"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/cb2004466"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573\u2010020\u201000093\u20101"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577\u2010022\u201000829\u20107"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2016.11.004"},{"key":"e_1_2_8_13_1","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2026102118","article-title":"Probing the Binding Specificities of Human Siglecs by Cell\u2010Based Glycan Arrays","volume":"118","author":"B\u00fcll C.","year":"2021","journal-title":"Proceedings National Academy of Science USA"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2015024118"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2019.01.002"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202514754"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.195925"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2018.02.005"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092\u20108674(01)00394\u20104"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1442"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102009\u201003\u2010210237"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.202401108"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cws077"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni0407\u2010339"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.691337"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010017\u201000662\u2010w"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/acschembio.4c00677"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.9b06406"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja907136d"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.200800757"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202111035"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201807742"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.joc.7b03117"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200705762"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.4c05090"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/tcr.202100080"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5SC04104J"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.9b08964"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.joc.1c01115"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacsau.4c00307"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.4c00363"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.4c14102"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.8b11005"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2022.106070"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41557\u2010024\u201001540\u2010x"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchem.2463"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.5c02356"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0040\u20104039(01)00157\u20105"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tetlet.2009.03.194"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.202500183"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.orglett.5c05037"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5QO00320B"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.4c16491"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tetasy.2016.06.010"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0040\u20104039(01)80526\u20108"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.5c14412"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja00327a021"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202405297"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi602543d"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010020\u201014794\u2010z"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6OB00586A"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202303832"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010021\u201023428\u2010x"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10719\u2010007\u20109063\u2010y"},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M000560200"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2011.09.027"},{"key":"e_1_2_8_67_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3737"},{"key":"e_1_2_8_68_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2107424118"},{"key":"e_1_2_8_69_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10719\u2010020\u201009912\u20104"},{"key":"e_1_2_8_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978\u20100\u201012\u2010800097\u20107.00003\u20108"},{"key":"e_1_2_8_71_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.accounts.3c00754"},{"key":"e_1_2_8_72_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41557\u2010018\u20100188\u20103"}],"container-title":["Angewandte Chemie International Edition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/anie.2218878","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/anie.2218878","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/anie.2218878","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T04:10:39Z","timestamp":1789531839000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/anie.2218878"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,15]]},"references-count":71,"alternative-id":["10.1002\/anie.2218878"],"URL":"https:\/\/doi.org\/10.1002\/anie.2218878","archive":["Portico"],"relation":{},"ISSN":["1433-7851","1521-3773"],"issn-type":[{"value":"1433-7851","type":"print"},{"value":"1521-3773","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,15]]},"assertion":[{"value":"2026-04-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2218878"}}