{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T07:49:46Z","timestamp":1791359386144,"version":"4.3.1"},"reference-count":68,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R35GM128704 to G. M"],"award-info":[{"award-number":["R35GM128704 to G. M"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["CHE\u20102045984 to G. M"],"award-info":[{"award-number":["CHE\u20102045984 to G. M"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Angew Chem Int Ed"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Overexpression of transmembrane Cu(I) P\u2010type ATPase pumps in cancer cells correlates with drug resistance towards chemotherapeutic Pt(II)\u2010based drugs, suggesting that they may facilitate the vesicular sequestration and cellular efflux of Pt(II)\u2010complexes. However, the ability of Cu(I)\u2010pumps to translocate Pt(II)\u2010drugs, including the molecular principles behind cargo recognition, remains elusive due to lack of tools to monitor in vitro turnover Pt(II)\u2010translocation events with transporters reconstituted into membrane\u2010mimetic systems. Here, we developed supramolecular mercaptosuccinic acid\u2010capped CdTe core\u2010type quantum dot\u2010encapsulated proteoliposome assemblies (MSA\u2010CdTe QD PLs) to demonstrate real\u2010time transporter\u2010mediated turnover translocation of Pt(II)\u2010complexes by Cu(I) P\u2010type ATPases. We coupled Pt(II)\u2010complex\u2010stimulated ATPase activity assays on the purified\n                    <jats:italic>E. coli<\/jats:italic>\n                    Cu(I)\u2010pump CopA with reconstitution into proteoliposomes and real\u2010time transport assays utilizing fluorescence detector probes (MSA\u2010CdTe QDs, pyranine, and oxonol VI) responsive to substrate, pH, and membrane potential, to demonstrate that Cu(I)\u2010pumps are electrogenic Pt(II)\u2010drug uniporters. Cu(I)\u2010pumps efficiently translocate aquated Pt(II)\u2010complexes with\n                    <jats:italic>cis<\/jats:italic>\n                    configuration (like cisplatin and carboplatin), but not transplatin, emphasizing how stereochemistry influences the Pt(II) transport properties. Additionally, the nature and kinetic lability of the carrier and leaving ligands were demonstrated to modulate the transport activity. Overall, MSA\u2010CdTe QD proteoliposome assemblies establish an extendable experimental platform to discover the Pt(II) transport properties of novel transporter classes.\n                  <\/jats:p>","DOI":"10.1002\/anie.2267666","type":"journal-article","created":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:53:31Z","timestamp":1791356011000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Supramolecular Cadmium\u2010Telluride Proteoliposomal Assemblies for Real\u2010Time Detection of Protein\u2010Catalyzed Pt(II)\u2010Complexes Transmembrane Translocation: Cu(I)\u2010Pumps Are Electrogenic Pt(II)\u2010Drug Uniporters"],"prefix":"10.1002","author":[{"given":"Madujika A. Horadigala","family":"Gamage","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry University of Texas at Dallas  Richardson Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9001-8719","authenticated-orcid":false,"given":"Alessandra","family":"Barbanente","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Bari Aldo Moro  Bari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadeesha T. Liyana","family":"Withanage","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry University of Texas at Dallas  Richardson Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yalini H.","family":"Wijesundara","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry University of Texas at Dallas  Richardson Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6400-8106","authenticated-orcid":false,"given":"Jeremiah J.","family":"Gassensmith","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry University of Texas at Dallas  Richardson Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8399-0964","authenticated-orcid":false,"given":"Fabio","family":"Arnesano","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Bari Aldo Moro  Bari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1790-6365","authenticated-orcid":false,"given":"Giovanni","family":"Natile","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Bari Aldo Moro  Bari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriele","family":"Meloni","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry University of Texas at Dallas  Richardson Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,6]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd1691"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00280\u2010016\u20102976\u2010z"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41568\u2010020\u201000308\u2010y"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.5b00597"},{"key":"e_1_2_8_6_1","doi-asserted-by":"crossref","first-page":"2467","DOI":"10.1021\/cr980421n","article-title":"Structure, Recognition, and Processing of Cisplatin\u2212DNA Adducts","volume":"99","author":"Jamieson E. 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Preparation and Ligand\u2010substitution Reaction of Di\u2010chloro(ethylenediamine)\u2010 and Dichloro(propylenediamine)\u2010gold(III) Complexes","volume":"7","author":"Annibale G.","year":"1978","journal-title":"Journal of the Chemical Society, Dalton Transactions"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jctc.5c01079"},{"key":"e_1_2_8_67_1","doi-asserted-by":"crossref","first-page":"2135","DOI":"10.1039\/dt9880002135","article-title":"Kinetics of the Displacement of Cyclobutane\u20101,1\u2010dicarboxylate From Diammine(cyclobutane\u20101,1\u2010dicarboxylato)Platinum(II) in Aqueous Solution","volume":"17","author":"Canovese L.","year":"1988","journal-title":"Journal of the Chemical Society, Dalton Transactions"},{"key":"e_1_2_8_68_1","doi-asserted-by":"crossref","first-page":"4821","DOI":"10.1039\/c0dt01758b","article-title":"Stability of Carboplatin and Oxaliplatin in Their Infusion Solutions Is Due to Self\u2010Association","volume":"40","author":"Di Pasqua A. 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