{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T15:54:16Z","timestamp":1791647656091,"version":"4.3.4"},"reference-count":70,"publisher":"Wiley","issue":"42","license":[{"start":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T00:00:00Z","timestamp":1787184000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T00:00:00Z","timestamp":1787184000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN\u20102024\u201005666"],"award-info":[{"award-number":["RGPIN\u20102024\u201005666"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN\u20102020\u201006401"],"award-info":[{"award-number":["RGPIN\u20102020\u201006401"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["VR2\u2010172718"],"award-info":[{"award-number":["VR2\u2010172718"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Angew Chem Int Ed"],"published-print":{"date-parts":[[2026,10,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Sensitive detection of viral RNA in biological samples remains challenging because of low viral loads, complex matrices, and RNA secondary structures. Here, we present a heat\u2010free, reverse\u2010transcription\u2010free lateral flow platform that converts structured viral RNA into visual signals through RNase I\u2010mediated primer generation, exponential rolling circle amplification (E\u2010RCA), and toehold\u2010mediated strand displacement (TMSD). The newly developed E\u2010RCA circuit uses a single additional primer, P2, which is extended on primary RCA products and then processed by \u03a629 DNA polymerase to generate a feedback primer, enabling rapid exponential amplification from a circular DNA template. RCA products and P2\u2010extended products subsequently trigger a newly designed TMSD module to release bridging DNA strands that generate visible lateral flow signals. Using SARS\u2010CoV\u20102 as a model, the assay achieved detection limits down to 500 aM, sample\u2010to\u2010result times of approximately 45 min, and 95%\u2013100% accuracy across four viral RNA regions in clinical saliva samples, providing a platform for rapid point\u2010of\u2010care RNA diagnostics.<\/jats:p>","DOI":"10.1002\/anie.3766984","type":"journal-article","created":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T12:01:11Z","timestamp":1787227271000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Coupling Exponential Rolling Circle Amplification to Lateral Flow Devices for Point\u2010of\u2010Care Detection of Viral RNA"],"prefix":"10.1002","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7066-9672","authenticated-orcid":false,"given":"Amal","family":"Mathai","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Biomedical Sciences McMaster University  Hamilton Ontario Canada"},{"name":"Biointerfaces Institute McMaster University  Hamilton Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monsur","family":"Ali","sequence":"additional","affiliation":[{"name":"Biointerfaces Institute McMaster University  Hamilton Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7533-6743","authenticated-orcid":false,"given":"Yingfu","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biomedical Sciences McMaster University  Hamilton Ontario Canada"},{"name":"Biointerfaces Institute McMaster University  Hamilton Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3461-9824","authenticated-orcid":false,"given":"John D.","family":"Brennan","sequence":"additional","affiliation":[{"name":"Biointerfaces Institute McMaster University  Hamilton Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,20]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1586\/14737159.7.6.775"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg.2016.10"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002311"},{"key":"e_1_2_9_5_1","first-page":"23","article-title":"Detection of 2019 Novel Coronavirus (2019\u2010nCoV) by Real\u2010Time RT\u2010PCR","volume":"25","author":"Corman V. M.","year":"2020","journal-title":"Euro Surveillance"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12275\u2010015\u20104656\u20109"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.57"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.12.e63"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0040204"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2015.245829"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1039\/b921140c"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2lc40100b"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.5b00428"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/20.7.1691"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/15257770701845204"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaq0179"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1039\/D2CS00613H"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200705982"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3cs60439j"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph11020035"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2021.02.007"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201700054"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2004.10.019"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2018.03.065"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13206\u2010016\u20100402\u20106"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.202300075"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010017\u201006594\u20101"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8CC00093J"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn1014"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2020.12.062"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202507973"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202525591"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2017.10.001"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274\u2010019\u20102601\u20105"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0278869"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2024.1379939"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13930"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25084237"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967\u2010025\u201006642\u20109"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2025.107768"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1039\/D4SC02648A"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.3c03995"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2022.107551"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2024.111313"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hal.2019.03.015"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2024.126441"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2025.117831"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1AN00399B"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snr.2025.100363"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9SC04738G"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2018.03.067"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi800746p"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.9.2519"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.202000245"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c03740"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1227\/neu.0000000000002914"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.3390\/bios12090739"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202509598"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202307451"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2023.105419"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2024.116421"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.01630\u201024"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010022\u201006642\u20105"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0243183"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.medj.2020.12.010"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1039\/D2AN00263A"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05448"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202112346"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201403222"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5SC04184H"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201600643"}],"container-title":["Angewandte Chemie International Edition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/anie.3766984","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/anie.3766984","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/anie.3766984","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T15:47:48Z","timestamp":1791647268000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/anie.3766984"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,20]]},"references-count":70,"journal-issue":{"issue":"42","published-print":{"date-parts":[[2026,10,12]]}},"alternative-id":["10.1002\/anie.3766984"],"URL":"https:\/\/doi.org\/10.1002\/anie.3766984","archive":["Portico"],"relation":{},"ISSN":["1433-7851","1521-3773"],"issn-type":[{"value":"1433-7851","type":"print"},{"value":"1521-3773","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,20]]},"assertion":[{"value":"2026-05-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e3766984"}}