{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T18:55:10Z","timestamp":1789498510262,"version":"build-2803163510"},"reference-count":55,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Angew Chem Int Ed"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>A multipurpose electroanalytical biotechnology to advance the role of atypical G\u2010quadruplex (G4) structures as emerging, multifunctional DNA biomarkers is reported. The technology presented enables determination, selective isolation, and in\u2010depth characterization of G4 motifs from liquid and solid biopsy samples obtained from oncology patients. The strategy relies on magnetic immunocaptors operating in a competitive assay between endogenous G4 targets and a homologous biotinylated G4 sequence labeled with an enzyme conjugate, coupled with amperometric transduction at screen\u2010printed electrodes. Beyond G4 determination and evaluation of the interaction of G4\u2010ligands using electroanalytical transduction, the strategy allows efficient G4 capture, facilitating their downstream structural and molecular characterization using complementary omics techniques. The analytical performance is validated in synthetic models and clinically relevant samples from colorectal cancer (CRC) patients, demonstrating the direct detection of G4 structures in plasma from oncology patients without prior nucleic acid extraction. Furthermore, by integrating magnetic immunocapture with PCR and DNA sequencing, and advanced proteomics, we confirm the selective isolation of endogenous G4\u2010forming DNA regions and show that CRC induces a stage\u2010dependent remodeling of the plasma G4 protein interactome beyond global G4 level changes, respectively. Notably, we identified a CRC\u2010associated G4\u2010binding protein linked to poor survival.<\/jats:p>","DOI":"10.1002\/anie.3768019","type":"journal-article","created":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T12:54:42Z","timestamp":1786452882000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Decoding Atypical G\u2010Quadruplexes and Their Interactome in Liquid Biopsies via Integrated Electroanalytical\u2013Proteomic Technologies"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-0785-1495","authenticated-orcid":false,"given":"Andrea","family":"Cabrero\u2010Mart\u00edn","sequence":"first","affiliation":[{"name":"Analytical Chemistry Department, Faculty of Chemical Sciences University Complutense of Madrid  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8865-1531","authenticated-orcid":false,"given":"V\u00edctor","family":"Ruiz\u2010Valdepe\u00f1as Montiel","sequence":"additional","affiliation":[{"name":"Analytical Chemistry Department, Faculty of Chemical Sciences University Complutense of Madrid  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5995-2677","authenticated-orcid":false,"given":"Javier","family":"Vel\u00e1zquez\u2010Guti\u00e9rrez","sequence":"additional","affiliation":[{"name":"Chronic Disease Programme, UFIEC Institute of Health Carlos III  Madrid, Majadahonda Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5141-0454","authenticated-orcid":false,"given":"Ana","family":"Montero\u2010Calle","sequence":"additional","affiliation":[{"name":"Chronic Disease Programme, UFIEC Institute of Health Carlos III  Madrid, Majadahonda Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda","family":"Garranzo\u2010Asensio","sequence":"additional","affiliation":[{"name":"Chronic Disease Programme, UFIEC Institute of Health Carlos III  Madrid, Majadahonda Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3626-9154","authenticated-orcid":false,"given":"A. Julio","family":"Reviejo","sequence":"additional","affiliation":[{"name":"Analytical Chemistry Department, Faculty of Chemical Sciences University Complutense of Madrid  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2271-1383","authenticated-orcid":false,"given":"Jos\u00e9 M.","family":"Pingarr\u00f3n","sequence":"additional","affiliation":[{"name":"Analytical Chemistry Department, Faculty of Chemical Sciences University Complutense of Madrid  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3539-7469","authenticated-orcid":false,"given":"Rodrigo","family":"Barderas","sequence":"additional","affiliation":[{"name":"Chronic Disease Programme, UFIEC Institute of Health Carlos III  Madrid, Majadahonda Spain"},{"name":"CIBER of Frailty and Healthy Aging, CIBERFES Institute of Health Carlos III  Madrid, Majadahonda Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9928-6613","authenticated-orcid":false,"given":"Susana","family":"Campuzano","sequence":"additional","affiliation":[{"name":"Analytical Chemistry Department, Faculty of Chemical Sciences University Complutense of Madrid  Madrid Spain"},{"name":"CIBER of Frailty and Healthy Aging, CIBERFES Institute of Health Carlos III  Madrid, Majadahonda Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,11]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591\u2010024\u201003046\u2010y"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010024\u201001823\u20102"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5c01450"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41576\u2010022\u201000539\u20109"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216\u2010025\u201006177\u20104"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.7203\/metode.12.16505"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008\u20105472.CAN\u201022\u20103089"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchem.1548"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0102711"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2022.959258"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.21037\/jgo\u201024\u201026"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5c02715"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00249\u2010025\u201001772\u2010w"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.0c11708"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.182256799"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059\u2010025\u201003678\u20104"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2025.103917"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomedchemau.4c00092"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2023.1136251"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.adr0493"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42004\u2010025\u201001462\u2010w"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010020\u201016168\u2010x"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2119235119"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2017.150"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41588\u2010020\u20100672\u20108"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2023.06.007"},{"key":"e_1_2_8_28_1","doi-asserted-by":"crossref","unstructured":"R. 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