{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T16:00:32Z","timestamp":1790438432514,"version":"4.1.0"},"reference-count":50,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T00:00:00Z","timestamp":1790380800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T00:00:00Z","timestamp":1790380800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R35GM128747"],"award-info":[{"award-number":["R35GM128747"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Angew Chem Int Ed"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Covalent conjugation of ligand molecules to force sensors provides a powerful approach to image receptor\u2010transmitted cellular forces. While this strategy is effective for small\u2010molecule ligands, the conjugation process can damage large protein ligands. To address this challenge, we developed a modular mechano\u2010fluorescent coating (mMFC) that enables receptor\u2010specific force imaging using widely available biotinylated ligands. The mMFC construct consists of a poly\u2010L\u2010lysine (PLL) backbone for substrate adsorption, polyethylene glycol (PEG) grafts to prevent nonspecific binding, and DNA\u2010based integrative tension sensors (ITS) as force\u2010reporting units. Benefiting from copper\u2010free click chemistry, the mMFC is synthesized via a simple one\u2010pot reaction. During application, the mMFC is adsorbed onto substrates, and biotinylated ligands are subsequently linked to the mMFC construct via biotin\u2010avidin interactions, where the ITS reports receptor\u2010transmitted forces by fluorescence. By testing HeLa cells, platelets, and human T lymphocytes on mMFC platforms, we imaged cellular forces transmitted through RGD peptide, latency\u2010associated peptide (LAP), ICAM\u20101, and anti\u2010CD3, which target integrins, LFA\u20101, and T cell receptor (TCR) complex, respectively. We further demonstrated molecular force calibration and single\u2010molecule force imaging of these receptors using the mMFC platform. Overall, the mMFC provides a modular, convenient, and robust platform for investigating mechanotransduction across diverse receptors in various cell types.<\/jats:p>","DOI":"10.1002\/anie.4231597","type":"journal-article","created":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T15:30:33Z","timestamp":1790436633000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Imaging and Calibration of Receptor\u2010Specific Cellular Forces Using Biotinylated Ligands"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1390-133X","authenticated-orcid":false,"given":"Vivek","family":"Pandey","sequence":"first","affiliation":[{"name":"Hoxworth Center College of Medicine University of Cincinnati  Cincinnati Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7770-9876","authenticated-orcid":false,"given":"Ying","family":"Tu","sequence":"additional","affiliation":[{"name":"Randox Laboratories Ltd.  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