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However, inherent limitations in ionisation efficiencies limit the scope of analyte detection. Currently, methods to enhance molecular coverage have focused on chemical derivatisation of target analyte classes or post\u2010ionisation techniques, namely, laser post\u2010ionisation (MALDI\u20102). Previously, we observed that under reduced laser fluence conditions, MALDI\u20102 can be utilised to selectively enhance photoionisation of aromatic analytes. Leveraging these observations, we have conducted a proof\u2010of\u2010concept study to establish a conceptually novel class of derivatisation reagents, employing a tocopherol active chromophore\n                    <jats:italic>O<\/jats:italic>\n                    \u2010linked succinimide (TAC\/OS) for the selective derivatisation of primary amines. Using TAC\/OS, amino metabolites were selectively detected as the derivatised radical cations in the presence of MALDI\u20102. Across three tissue types, over 30 metabolites were detected and localised, including 20 amino acids alongside neurotransmitters such as \u03b3\u2010aminobutyric acid (GABA) and dopamine. Imaging at 10\u00a0\u00b5m pixel size revealed minimal delocalisation, allowing for MSI at near single\u2010cell resolution. Furthermore, we demonstrated the ability of the developed protocol on late\u2010stage pancreatic cancer xenografts.\n                  <\/jats:p>","DOI":"10.1002\/anie.4492989","type":"journal-article","created":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T10:52:37Z","timestamp":1783507957000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Beyond the Fixed Charge: A New On\u2010Tissue Derivatisation Strategy Employing Photoionisable Chromophores for Mass Spectrometry Imaging of Amino\u2010Containing Metabolites"],"prefix":"10.1002","volume":"65","author":[{"given":"Sofia R.","family":"McGowan","sequence":"first","affiliation":[{"name":"School of Science, Molecular Horizons Research Institute University of Wollongong Wollongong New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew J.","family":"Tague","sequence":"additional","affiliation":[{"name":"School of Science, Molecular Horizons Research Institute University of Wollongong Wollongong New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashna A.","family":"Kumar","sequence":"additional","affiliation":[{"name":"School of Science, Molecular Horizons Research Institute University of Wollongong Wollongong New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Ranson","sequence":"additional","affiliation":[{"name":"School of Science, Molecular Horizons Research Institute University of Wollongong Wollongong New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9963-5924","authenticated-orcid":false,"given":"Christopher J. 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