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Integrating such synthetic organelles into protocell carriers remains a key challenge. Here, we introduce a method to assemble functional phase\u2010separated organelles within liposome protocells. Pre\u2010engineered nucleic acids are encapsulated with ligase in locked\u2010DNA\u2010nanopore modified protocells. Upon nanopore unlocking and Mg\n                    <jats:sup>2+<\/jats:sup>\n                    influx, the nucleic acid constituents ligate into programmable polymer chains that crosslink into barcode\u2010modified condensates. Photoresponsive, caged nucleic acids hybridize with barcode tethers on two distinct organelles, forming a functional two\u2010organelle system in the protocells. Light\u2010induced uncaging releases an information\u2010transfer strand from one organelle, triggering intercommunication and reconfiguration of the partner organelle. By predesigning organelle compositions and transfer strands, the emergence of catalytic DNAzymes or transcriptional machinery in the organelle\/protocell assemblies is demonstrated, resulting in dynamic structural reconfiguration of the organelles.\n                  <\/jats:p>","DOI":"10.1002\/anie.4889049","type":"journal-article","created":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T13:34:36Z","timestamp":1777642476000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Photoactivated Signaling Networks using DNA\u2010Based Synthetic Organelles as Biomimetic Protocells"],"prefix":"10.1002","volume":"65","author":[{"given":"Huiying","family":"Xue","sequence":"first","affiliation":[{"name":"State Key Laboratory of Geomicrobiology and Environmental Changes Faculty of Materials Science and Chemistry China University of Geosciences  Wuhan 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