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Recently, core\u2010xylosylated N\u2010glycans have attracted considerable attention in the medical field, as they could elicit strong immune responses. Notably, antibodies targeting core\u2010xylosylated N\u2010glycans could kill\n                    <jats:italic>Schistosoma<\/jats:italic>\n                    in vitro, and thus process potentials for protecting host from\n                    <jats:italic>Schistosoma<\/jats:italic>\n                    infections. However, the structural complexity has posed significant challenges to access structure\u2010defined core\u2010xylosylated N\u2010glycans. Here, a powerful synthetic platform enabling the efficient preparation of core\u2010xylosylated N\u2010glycans is described. The strategy is based on de novo biosynthetic pathways to efficiently generate a sufficient quantity of N\u2010glycan core precursors, coupled with the use of a robust \u03b21,2\u2010Xylosyltransferase to install core xylose in a stereoselective and regioselective manner. This approach further leverages diversity\u2010oriented synthesis to systematically construct a comprehensive core\u2010xylosylated N\u2010glycan library, encompassing a broad range of structurally diverse N\u2010glycans through enzymatic extension strategy. With the glycan array made from this glycan library, we investigated the binding activities of the antibodies against\n                    <jats:italic>Schistosoma japonicum<\/jats:italic>\n                    , which has caused widespread infections in Asia. In addition to the synthetic breakthroughs, this work also provides an insightful understanding of the structure\u2013function relationships of core\u2010xylosylated N\u2010glycans, which will advance the rational design of carbohydrate\u2010based vaccine against\n                    <jats:italic>Schistosoma<\/jats:italic>\n                    infections.\n                  <\/jats:p>","DOI":"10.1002\/anie.5002594","type":"journal-article","created":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T16:47:10Z","timestamp":1778863630000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A De Novo Chemoenzymatic Assembly Strategy Enables the Efficient Preparation of a Large Core\u2010Xylosylated N\u2010Glycan Library for Probing Antibodies Against\n                    <i>Schistosoma japonicum<\/i>"],"prefix":"10.1002","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5627-253X","authenticated-orcid":false,"given":"Fangyu","family":"Wei","sequence":"first","affiliation":[{"name":"State Key Laboratory of Chemical Biology Shanghai Institute of Materia Medica Chinese Academy of Sciences  Shanghai China"},{"name":"Department of Carbohydrate\u2010Based Drug Research Center Shanghai Institute of Materia Medica Chinese Academy of Sciences  Shanghai China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Carbohydrate\u2010Based Drug Research Center Shanghai Institute of Materia Medica Chinese Academy of Sciences  Shanghai China"},{"name":"Zhongshan Institute for Drug Discovery Shanghai Institute of Materia Medica Chinese Academy of Sciences  Zhongshan Guangdong Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dingyi","family":"Mao","sequence":"additional","affiliation":[{"name":"National Health Commission Key Laboratory of Parasitic Disease Control and Prevention Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology Jiangsu Provincial Medical Key Laboratory Jiangsu Institute of Parasitic Diseases  Wuxi Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1489-9080","authenticated-orcid":false,"given":"Yang","family":"Dai","sequence":"additional","affiliation":[{"name":"National Health Commission Key Laboratory of Parasitic Disease Control and Prevention Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology Jiangsu Provincial Medical Key Laboratory Jiangsu Institute of Parasitic Diseases  Wuxi Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0128-655X","authenticated-orcid":false,"given":"Jiabin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Carbohydrate\u2010Based Drug Research Center Shanghai Institute of Materia Medica Chinese Academy of Sciences  Shanghai China"},{"name":"University of Chinese Academy of Sciences  Beijing China"},{"name":"Zhongshan Institute for Drug Discovery Shanghai Institute of Materia Medica Chinese Academy of Sciences  Zhongshan Guangdong Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9187-7999","authenticated-orcid":false,"given":"Liuqing","family":"Wen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Chemical Biology Shanghai Institute of Materia Medica Chinese Academy of Sciences  Shanghai China"},{"name":"Department of Carbohydrate\u2010Based Drug Research Center Shanghai Institute of Materia Medica Chinese Academy of Sciences  Shanghai China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,15]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.8b00066"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2020.00513"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwg024"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014\u20105793(04)00150\u20104"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000096114"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cww023"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms161226122"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.15.11451"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014\u20104894(03)00110\u20103"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.11.4159"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071\u2010015\u20100842\u20101"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2023.1142620"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/trstmh\/trab002"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0009373"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(09)60233\u20106"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2438"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.351.6268.16"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010019\u201009752\u20103"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202513638"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202206802"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.bioconjchem.3c00302"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/acschembio.1c00597"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.3c02976"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2009.02.037"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1158\/2326\u20106066.CIR\u201020\u20100264"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.6283"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwn071"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1039\/D4OB01006J"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchem.2463"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja409097c"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.5c02356"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1236231"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41557\u2010018\u20100188\u20103"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja307628w"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja401385v"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201604190"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201807742"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/ange.202111035"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200500777"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja021288q"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja208390n"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.1c03174"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chempr.2024.05.006"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.5c11949"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1021\/cb501023u"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1021\/acschembio.6b00265"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202105647"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M115.048280"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01349\u201015"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carres.2017.10.001"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6OB01982J"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202211032"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1039\/D2OB00615D"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014\u20105793(00)01443\u20105"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiosc.2011.09.011"},{"key":"e_1_2_8_57_1","doi-asserted-by":"p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