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Mechanistically, CO\n                    <jats:sub>2<\/jats:sub>\n                    diffuses in and reacts with MEA or hydroxide to form a mixture of carbamate and carbonate\/bicarbonate. Subsequently, CO\n                    <jats:sub>2<\/jats:sub>\n                    is released for storage or utilization via an energy\u2010intensive thermal solvent\u2010regeneration process. Here, we explored biocatalytic acceleration of the solvent regeneration step, which accounts for the dominant energy cost of carbon dioxide removal (CDR) processes. We conducted a sequence mining campaign based on urethane\u2010degrading Amidase Signature superfamily enzymes and discovered amidases that hydrolyze MEA carbamate, thereby increasing the overall release rate of CO\n                    <jats:sub>2<\/jats:sub>\n                    . The most promising candidate, an amidase from\n                    <jats:italic>Parageobacillus caldoxylosilyticus<\/jats:italic>\n                    (PcAmd), showed good thermostability (\n                    <jats:italic>T<\/jats:italic>\n                    <jats:sub>m<\/jats:sub>\n                    around 70\u00b0C) and a specific activity against MEA carbamate of about 1 U\/mg (20 nKat\/mg). We found that PcAmd accelerated the regeneration of MEA sorbent. Specifically, PcAmd at 1\u00a0\u00b5M increased the initial CO\n                    <jats:sub>2<\/jats:sub>\n                    release rate by about 20%, and the time required to release 80% of the captured CO\n                    <jats:sub>2<\/jats:sub>\n                    was reduced by approximately half compared to enzyme\u2010free solutions. These results identified a novel potential of amidases in carbon capture and motivated further efforts to discover or engineer enzymes with better stability and activity for industrial CDR applications.\n                  <\/jats:p>","DOI":"10.1002\/anie.5406475","type":"journal-article","created":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T11:42:08Z","timestamp":1782474128000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Amidase\u2010Catalyzed Desorption of CO\n                    <sub>2<\/sub>\n                    Captured in Aqueous Monoethanolamine (MEA) Solutions"],"prefix":"10.1002","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7588-1653","authenticated-orcid":false,"given":"Yang","family":"Yang","sequence":"first","affiliation":[{"name":"Protein and Enzyme Technology Department of Biotechnology and Biomedicine Technical University of Denmark  Lyngby Denmark"},{"name":"The Novo Nordisk Foundation CO<sub>2<\/sub> Research Center (CORC) Aarhus University  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7454-6810","authenticated-orcid":false,"given":"Onur","family":"K\u0131rtel","sequence":"additional","affiliation":[{"name":"The Novo Nordisk Foundation Biotechnology Research Institute For the Green Transition Technical University of Denmark  Lyngby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silke Flindt","family":"Badino","sequence":"additional","affiliation":[{"name":"Protein and Enzyme Technology Department of Biotechnology and Biomedicine Technical University of Denmark  Lyngby Denmark"},{"name":"The Novo Nordisk Foundation CO<sub>2<\/sub> Research Center (CORC) Aarhus University  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lea Helena","family":"Strother","sequence":"additional","affiliation":[{"name":"The Novo Nordisk Foundation Biotechnology Research Institute For the Green Transition Technical University of Denmark  Lyngby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Rotilio","sequence":"additional","affiliation":[{"name":"Protein and Enzyme Technology Department of Biotechnology and Biomedicine Technical University of Denmark  Lyngby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerik Mathew Valera","family":"Lauridsen","sequence":"additional","affiliation":[{"name":"The Novo Nordisk Foundation CO<sub>2<\/sub> Research Center (CORC) Aarhus University  Aarhus Denmark"},{"name":"Department of Chemistry University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefanie","family":"Neun","sequence":"additional","affiliation":[{"name":"Novonesis  Lyngby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens Preben","family":"Morth","sequence":"additional","affiliation":[{"name":"Protein and Enzyme Technology Department of Biotechnology and Biomedicine Technical University of Denmark  Lyngby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6177-4569","authenticated-orcid":false,"given":"Ji\u2010Woong","family":"Lee","sequence":"additional","affiliation":[{"name":"The Novo Nordisk Foundation CO<sub>2<\/sub> Research Center (CORC) Aarhus University  Aarhus Denmark"},{"name":"Department of Chemistry University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ditte Hededam","family":"Welner","sequence":"additional","affiliation":[{"name":"The Novo Nordisk Foundation Biotechnology Research Institute For the Green Transition Technical University of Denmark  Lyngby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6185-0637","authenticated-orcid":false,"given":"Peter","family":"Westh","sequence":"additional","affiliation":[{"name":"Protein and Enzyme Technology Department of Biotechnology and Biomedicine Technical University of Denmark  Lyngby Denmark"},{"name":"The Novo Nordisk Foundation CO<sub>2<\/sub> Research Center (CORC) Aarhus University  Aarhus 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