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Vaccines are economical and effective tools against bacterial infection. Herein, we present the first total synthesis and immunological evaluation of the\n                    <jats:italic>E. coli<\/jats:italic>\n                    O29,\n                    <jats:italic>S. dysenteriae<\/jats:italic>\n                    serotype 11, and\n                    <jats:italic>P. vulgaris<\/jats:italic>\n                    O12 O\u2010antigen decasaccharides, pentasaccharides, and related oligosaccharide fragments. We found that the hexasaccharide, including two repeating units of the core trisaccharide, was the optimal epitope, which was conjugated with the carrier protein CRM197 to furnish a semisynthetic glycoconjugate vaccine. The glycoconjugate vaccine induced a robust antigen\u2010specific immune response, and the antibody sera could recognize the surface O\u2010antigens of\n                    <jats:italic>E. coli<\/jats:italic>\n                    O29. This glycoconjugate vaccine, including a common trisaccharide fragment, existed in many bacterial O\u2010antigens, which can be further used as a potential broad\u2010spectrum vaccine against\n                    <jats:italic>E. coli<\/jats:italic>\n                    ,\n                    <jats:italic>S. dysenteriae<\/jats:italic>\n                    ,\n                    <jats:italic>P. vulgaris<\/jats:italic>\n                    , and\n                    <jats:italic>Cronobacter malonaticus (C. malonaticus)<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1002\/anie.5838072","type":"journal-article","created":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T07:58:48Z","timestamp":1782201528000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Chemical Synthesis and Immunological Evaluation of\n                    <i>Escherichia coli<\/i>\n                    O29,\n                    <i>Shigella dysenteriae<\/i>\n                    Serotype 11, and\n                    <i>Proteus vulgaris<\/i>\n                    O12 O\u2010Antigen Oligosaccharides"],"prefix":"10.1002","volume":"65","author":[{"given":"Xinxin","family":"Li","sequence":"first","affiliation":[{"name":"National Research Centre for Carbohydrate Synthesis College of Chemistry and Materials Jiangxi Normal University  Nanchang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaotong","family":"Wang","sequence":"additional","affiliation":[{"name":"Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism Shanghai Key Laboratory of New Drug Design Engineering Research Center of Pharmaceutical Process Chemistry Ministry of Education East China University of Science and Technology  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Deng","sequence":"additional","affiliation":[{"name":"National Research Centre 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Jiangxi Normal University  Nanchang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Wang","sequence":"additional","affiliation":[{"name":"National Research Centre for Carbohydrate Synthesis College of Chemistry and Materials Jiangxi Normal University  Nanchang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liming","family":"Wang","sequence":"additional","affiliation":[{"name":"National Research Centre for Carbohydrate Synthesis College of Chemistry and Materials Jiangxi Normal University  Nanchang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4113-506X","authenticated-orcid":false,"given":"Xinshan","family":"Ye","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical Sciences and Chemical Biology Center Peking University  Beijing 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