{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T15:54:01Z","timestamp":1791647641012,"version":"4.3.4"},"reference-count":52,"publisher":"Wiley","issue":"42","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001824","name":"Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["25\u201315592S"],"award-info":[{"award-number":["25\u201315592S"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Angew Chem Int Ed"],"published-print":{"date-parts":[[2026,10,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>We designed and synthesized 2\u2019\u2010deoxyribonucleoside triphosphates (dNTPs) bearing chlorohexyl and biotin modifications attached to position 7 of 7\u2010deazaadenine or to position 5 of cytosine. The modified dNTPs served as substrates for KOD XL DNA polymerase in the enzymatic construction of DNA bearing either one or a combination of the two modifications. The chlorohexyl\u2010linked DNA underwent covalent reactions with halotag protein, while the biotin\u2010linked DNA formed stable non\u2010covalent complexes with monomeric streptavidin. The dually modified DNA bearing both modifications at different distances apart from each other were used for sequence\u2010specific, spatially defined display of both halotag and streptavidin proteins. The study provides the foundation for display of proteins on DNA rulers.<\/jats:p>","DOI":"10.1002\/anie.6259985","type":"journal-article","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T14:29:28Z","timestamp":1787149768000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Biotin\u2010 and Chloroalkane\u2010Modified Nucleotides for Dual Display of Proteins on DNA Through Streptavidin and Halotag Binding"],"prefix":"10.1002","volume":"65","author":[{"given":"Giammarco","family":"Liuni","sequence":"first","affiliation":[{"name":"Institute of Organic Chemistry and Biochemistry Czech Academy of Sciences  Prague Czech Republic"},{"name":"Department of Organic Chemistry Faculty of Science Charles University  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lenka Po\u0161tov\u00e1","family":"Slav\u011bt\u00ednsk\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Organic Chemistry and Biochemistry Czech Academy of Sciences  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miroslav","family":"H\u00e1jek","sequence":"additional","affiliation":[{"name":"Institute of Organic Chemistry and Biochemistry Czech Academy of Sciences  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronika","family":"Raindlov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Organic Chemistry and Biochemistry Czech Academy of Sciences  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1113-2047","authenticated-orcid":false,"given":"Michal","family":"Hocek","sequence":"additional","affiliation":[{"name":"Institute of Organic Chemistry and Biochemistry Czech Academy of Sciences  Prague Czech Republic"},{"name":"Department of Organic Chemistry Faculty of Science Charles University  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,19]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsmaterialslett.4c01209"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijch.201200100"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0CS00518E"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2019.06.006"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.7b09967"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200903328"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1039\/C0SC00565G"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.202200561"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.bioconjchem.7b00382"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/app12031717"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom12101523"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2022.08.004"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202314452"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202215023"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201005931"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nnano.2009.50"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja0383367"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacsau.1c00387"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.accounts.9b00195"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42004\u2010024\u201001216\u20100"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.accounts.5b00544"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3cc41438h"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201906737"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202105126"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2019.07.007"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.202402151"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41557\u2010023\u201001196\u2010z"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42004\u2010024\u201001255\u20107"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.24320"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.24605"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/cb800025k"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.11.6633"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.9.2593"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchem.2493"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.bioconjchem.3c00064"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.200700220"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja403323y"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1021\/acschembio.5b00442"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1021\/ol070588f"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.201200306"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1039\/B505737J"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja051725b"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202307548"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.0c00928"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.202000037"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201600039"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.119.3.493"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.75917"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201047444\u20109"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cclet.2017.08.003"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5b00140"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"}],"container-title":["Angewandte Chemie International Edition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/anie.6259985","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/anie.6259985","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/anie.6259985","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T15:46:44Z","timestamp":1791647204000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/anie.6259985"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,19]]},"references-count":52,"journal-issue":{"issue":"42","published-print":{"date-parts":[[2026,10,12]]}},"alternative-id":["10.1002\/anie.6259985"],"URL":"https:\/\/doi.org\/10.1002\/anie.6259985","archive":["Portico"],"relation":{},"ISSN":["1433-7851","1521-3773"],"issn-type":[{"value":"1433-7851","type":"print"},{"value":"1521-3773","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,19]]},"assertion":[{"value":"2026-04-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e6259985"}}