{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T14:09:41Z","timestamp":1785247781721,"version":"3.55.0"},"reference-count":53,"publisher":"Wiley","issue":"31","license":[{"start":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T00:00:00Z","timestamp":1781136000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T00:00:00Z","timestamp":1781136000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Angew Chem Int Ed"],"published-print":{"date-parts":[[2026,7,27]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Nucleoside\u20105\u2032\u2010triphosphates (NTPs) are important building blocks for nucleic acids, but the efficiency of their chemical synthesis is limited by the low yield of the final 5\u2032\u2010triphosphorylation reaction. Here, we present an enzymatic cascade reaction involving just two enzymes that enable nucleosides\u2010to\u2010NTPs conversion for all canonical rNTPs and dNTPs. The cascade is used in a single one\u2010pot operation driven by cost\u2010efficient sodium polyphosphate (polyP). The broad\u2010spectrum deoxynucleoside kinase (Dm\u2010dNK) from\n                    <jats:italic>Drosophila melanogaster<\/jats:italic>\n                    converts nucleosides into nucleoside\u20105\u2032\u2010monophosphates (NMPs) using ATP. The polyphosphate kinase family 2, class III (EbPPK) from an\n                    <jats:italic>Erysipelotrichaceae<\/jats:italic>\n                    bacterium simultaneously recycles ATP required by Dm\u2010dNK and converts the NMPs to NTPs in a polyP\u2010dependent reaction. Importantly, only trace amounts of catalytic ATP (0.0001\u00a0equivalents or less) are required. In addition to canonical NTPs, the cascade can generate ribose\u2010 and base\u2010modified NTP analogues. This is demonstrated through the synthesis of a set of eleven modified nucleotides, including the four 2\u2032\u2010fluoro\u20102\u2032\u2010dNTPs and nucleotides with 2,6\u2010diaminopurine as the nucleobase. Production of an essential component for mRNA therapeutics,\n                    <jats:italic>N<\/jats:italic>\n                    1\u2010methyl\u2010\u03a8TP (m\n                    <jats:sup>1<\/jats:sup>\n                    \u03a8TP), is demonstrated on a milligram scale.\n                  <\/jats:p>","DOI":"10.1002\/anie.6720227","type":"journal-article","created":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T07:57:12Z","timestamp":1781164632000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Universal Nucleoside\u2010to\u2010Nucleoside\u20105\u2032\u2010Triphosphate Enzyme Cascade Driven by Polyphosphate"],"prefix":"10.1002","volume":"65","author":[{"given":"Jonathan P.","family":"Suess","sequence":"first","affiliation":[{"name":"Institute of Biochemistry University of M\u00fcnster  M\u00fcnster Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3625-7612","authenticated-orcid":false,"given":"Nicolas V.","family":"Cornelissen","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry University of M\u00fcnster  M\u00fcnster Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,11]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2008.200"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2015.08.051"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010022\u201001007\u2010w"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio839"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.202200006"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.5c14653"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trechm.2025.11.006"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.202500650"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41557\u2010025\u201001925\u20106"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.add5892"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587\u2010024\u201002244\u2010w"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.202500241"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1039\/d5cc02749g"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/acschembio.5c00692"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/ceur.202500186"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaf155"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0040\u20104039(01)89915\u20109"},{"key":"e_1_2_8_19_1","first-page":"131","article-title":"A New Route to Nucleoside 5'\u2010Triphosphates","volume":"16","author":"Ludwig J.","year":"1981","journal-title":"Acta Biochimica Et Biophysica; 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