{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T18:56:55Z","timestamp":1791226615830,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"41","license":[{"start":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T00:00:00Z","timestamp":1786060800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T00:00:00Z","timestamp":1786060800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Angew Chem Int Ed"],"published-print":{"date-parts":[[2026,10,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Condensation (C) domains in nonribosomal peptide synthetase (NRPS) pathways exhibit versatile functions that drive biosynthetic and chemical novelty. Through genome mining for atypical C domains, we identified a hybrid NRPS\/polyketide synthase (PKS) biosynthetic gene cluster (\n                    <jats:italic>mxg<\/jats:italic>\n                    ) from\n                    <jats:italic>Cystobacterineae<\/jats:italic>\n                    sp. MCy9003 and discovered myxoglucamides, a family of glycolipopeptides featuring an unprecedented vinyl\u2010substituted \u03b3\u2010amino acid bearing an \u03b1\u2010hydroxy\/\u03b1\u2010ketoamide functionality. Heterologous expression of the promoter\u2010refactored pathway revealed new\n                    <jats:italic>O<\/jats:italic>\n                    \u2010acylated myxoglucamides, and subsequent studies unveiled the C domain\u2010like enzyme MxgH as a promiscuous\n                    <jats:italic>O<\/jats:italic>\n                    \u2010acyltransferase decorating the glucose moiety with short\u2010chain acyl groups. Biosynthetic investigations demonstrated that the unusual \u03b3\u2010amino acid originates from\n                    <jats:sc>l<\/jats:sc>\n                    \u2010glutamate. Completion of the cryptic \u03b2\u2010hydroxylation of peptidyl carrier protein\u2010tethered glutamate by the \u03b1\u2010ketoglutarate\u2010dependent dioxygenase Ox\n                    <jats:sub>MxgA<\/jats:sub>\n                    occurs only concomitantly with upstream chain extension, revealing a bidirectional checkpoint for substrate fidelity. Unexpectedly, the C\u2010domain\u2010like interface domain I\n                    <jats:sub>MxgB<\/jats:sub>\n                    is dispensable for this coupled transformation. Mutational analysis of the FMN\u2010dependent monooxygenase encoded by\n                    <jats:italic>mxgE<\/jats:italic>\n                    , together with characterization of a shunt metabolite, supported its role in \u03b1\u2010oxidation for \u03b1\u2010hydroxy\/\u03b1\u2010ketoamide formation during \u03b3\u2010amino acid assembly. Together, these findings uncover an unrecognized biosynthetic logic for generating vinyl\u2010substituted, \u03b1\u2010oxidized \u03b3\u2010amino acids and substantially expand the functional repertoire of NRPS\/PKS assembly lines.\n                  <\/jats:p>","DOI":"10.1002\/anie.6995139","type":"journal-article","created":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T13:26:59Z","timestamp":1786109219000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Atypical Condensation Domains Guide Discovery and Illuminate Biosynthesis of Myxoglucamides Featuring Vinyl\u2010Substituted, \u03b1\u2010Oxidized \u03b3\u2010Amino Acids"],"prefix":"10.1002","volume":"65","author":[{"given":"Tingting","family":"Wang","sequence":"first","affiliation":[{"name":"Helmholtz Institute for Pharmaceutical Research Saarland (HIPS) Helmholtz Centre for Infection Research (HZI) and Department of Pharmacy Saarland University  Saarbr\u00fccken Germany"},{"name":"Helmholtz International Lab for Anti\u2010Infectives Helmholtz Centre for Infection Research Braunschweig Germany"},{"name":"Helmholtz International Laboratory State Key Laboratory of Microbial Technology Shandong University  Qingdao P. 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