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Herein, we report a facile TK\u2010anchored enzyme condensate strategy to overcome this bottleneck. Specifically, the UTX\u2010derived intrinsically disordered region (IDR) fused with target enzymes mediates liquid\u2013liquid phase separation to form enzyme\u2010rich condensates, which then stably anchor on TK membranes and spatially confine catalysis at the NADPH\u2010generating membrane interface. Taking carbonyl reductase\n                    <jats:italic>Sm<\/jats:italic>\n                    CR\n                    <jats:sub>V4<\/jats:sub>\n                    and ene\u2010reductase\n                    <jats:italic>Ss<\/jats:italic>\n                    ER\n                    <jats:sub>A314S<\/jats:sub>\n                    as model enzymes, the TK\u2013enzyme hybrid condensates exhibit prominently improved catalytic performance, especially under NADPH regeneration\u2010limited conditions, relative to freely dispersed enzyme systems. The TK\u2013UTX\u2010\n                    <jats:italic>Sm<\/jats:italic>\n                    CR\n                    <jats:sub>V4<\/jats:sub>\n                    hybrid improves the total product yield up to 3.1\u2010fold, and the TK\u2013UTX\u2010\n                    <jats:italic>Ss<\/jats:italic>\n                    ER\n                    <jats:sub>A314S<\/jats:sub>\n                    hybrid reaches a 2.9\u2010fold higher conversion within 20\u00a0min. Coarse\u2010grained molecular dynamics simulations validate a dynamic multivalent interfacial anchoring mode: the IDR domain mediates stable membrane binding via high\u2010frequency residue\u2013lipid interactions dominated by short\u2010range Lennard\u2013Jones forces (with auxiliary electrostatic contributions), while the enzyme domain remains solvent\u2010exposed. This work establishes a feasible strategy that physically links light\u2010driven NADPH recycling with enzymatic catalysis, offering a general approach for constructing high\u2010efficiency artificial photosynthesis systems.\n                  <\/jats:p>","DOI":"10.1002\/anie.7079393","type":"journal-article","created":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T07:14:52Z","timestamp":1792826092000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Liquid\u2010Liquid Phase Separation Modulated Thylakoid\u2013Enzyme Interfacial Coupling for Enhanced Light\u2010Powered Biocatalysis"],"prefix":"10.1002","author":[{"given":"Jiayi","family":"Zhang","sequence":"first","affiliation":[{"name":"Shanghai Collaborative Innovation Center for Biomanufacturing East China University of Science and Technology  Shanghai 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