{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T14:09:42Z","timestamp":1785247782781,"version":"3.55.0"},"reference-count":59,"publisher":"Wiley","issue":"31","license":[{"start":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T00:00:00Z","timestamp":1780444800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T00:00:00Z","timestamp":1780444800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22377110"],"award-info":[{"award-number":["22377110"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82402749"],"award-info":[{"award-number":["82402749"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U23A20531"],"award-info":[{"award-number":["U23A20531"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22122409"],"award-info":[{"award-number":["22122409"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006407","name":"Natural Science Foundation of Henan Province","doi-asserted-by":"publisher","award":["252300421073"],"award-info":[{"award-number":["252300421073"]}],"id":[{"id":"10.13039\/501100006407","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011447","name":"Science and Technology Department of Henan Province","doi-asserted-by":"publisher","award":["252102311025"],"award-info":[{"award-number":["252102311025"]}],"id":[{"id":"10.13039\/501100011447","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Angew Chem Int Ed"],"published-print":{"date-parts":[[2026,7,27]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Highly sensitive spatial analysis of RNA mutations is essential for understanding cellular heterogeneity and disease mechanisms. Herein, we developed an integrated CRISPR\/Cas13a\u2010based nanoprobe system for rapid detection of RNA in tissue sections (\n                    <jats:styled-content>In<\/jats:styled-content>\n                    tegrated CRISPR\/\n                    <jats:styled-content>Cas<\/jats:styled-content>\n                    13a\u2010based RNA\n                    <jats:styled-content>R<\/jats:styled-content>\n                    apid\n                    <jats:styled-content>D<\/jats:styled-content>\n                    etection, InCasRD). Unlike conventional \u201calways\u2010on\u201d probes that rely on accumulated probe hybridization, InCasRD leverages the trans\u2010cleavage activity of Cas13a to achieve spatially confined signal amplification and a high signal\u2010to\u2010background ratio (SBR). Using InCasRD, we achieved imaging of multiple target RNAs in tumor cells within 0.5\u00a0h of incubation, including mRNA (\n                    <jats:italic>survivin<\/jats:italic>\n                    ), microRNA (miR\u201021), and circular RNA (circ1785). Furthermore, the engineered InCasRD system enabled mapping of RNA mutations, such as the\n                    <jats:italic>EGFR<\/jats:italic>\n                    L858R and ovarian tumor domain (\n                    <jats:italic>OTUD<\/jats:italic>\n                    ) single\u2010nucleotide variant (SNV, 23439980 G&gt;T), in tumor tissue sections, thereby facilitating clear tumor boundary delineation. Collectively, InCasRD is a powerful, one\u2010step tool for in situ RNA analysis with potential for diagnosis and precision medicine.\n                  <\/jats:p>","DOI":"10.1002\/anie.7088080","type":"journal-article","created":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T12:16:09Z","timestamp":1780488969000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["In Situ Amplified Mutational mRNA Imaging Using a Spatially Confined CRISPR Nanoplatform"],"prefix":"10.1002","volume":"65","author":[{"given":"Wen","family":"Zhao","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Sciences Key Laboratory of Advanced Drug Preparation Technologies Ministry of Education China Pingyuan Laboratory Tianjian Laboratory of Advanced Biomedical Sciences State Key Laboratory of Antiviral Drugs Zhengzhou University  Zhengzhou Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyue","family":"Zheng","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Key Laboratory of Advanced Drug Preparation Technologies Ministry of Education China Pingyuan Laboratory Tianjian Laboratory of Advanced Biomedical Sciences State Key Laboratory of Antiviral Drugs Zhengzhou University  Zhengzhou Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Li","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Key Laboratory of Advanced Drug Preparation Technologies Ministry of Education China Pingyuan Laboratory Tianjian Laboratory of Advanced Biomedical Sciences State Key Laboratory of Antiviral Drugs Zhengzhou University  Zhengzhou Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haojie","family":"Xie","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Key Laboratory of Advanced Drug Preparation Technologies Ministry of Education China Pingyuan Laboratory Tianjian Laboratory of Advanced Biomedical Sciences State Key Laboratory of Antiviral Drugs Zhengzhou University  Zhengzhou Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiyun","family":"Yu","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Key Laboratory of Advanced Drug Preparation Technologies Ministry of Education China Pingyuan Laboratory Tianjian Laboratory of Advanced Biomedical Sciences State Key Laboratory of Antiviral Drugs Zhengzhou University  Zhengzhou Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongyuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathogen Biology School of Basic Medicine Zhengzhou University  Zhengzhou P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yonghua","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Key Laboratory of Advanced Drug Preparation Technologies Ministry of Education China Pingyuan Laboratory Tianjian Laboratory of Advanced Biomedical Sciences State Key Laboratory of Antiviral Drugs Zhengzhou University  Zhengzhou Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunqing","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Breast Surgery The First Affiliated Hospital of Zhengzhou University  Zhengzhou Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenzhong","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Key Laboratory of Advanced Drug Preparation Technologies Ministry of Education China Pingyuan Laboratory Tianjian Laboratory of Advanced Biomedical Sciences State Key Laboratory of Antiviral Drugs Zhengzhou University  Zhengzhou Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9250-0067","authenticated-orcid":false,"given":"Hua","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Pathogen Biology School of Basic Medicine Zhengzhou University  Zhengzhou P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9528-0273","authenticated-orcid":false,"given":"Yanan","family":"Li","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Key Laboratory of Advanced Drug Preparation Technologies Ministry of Education China Pingyuan Laboratory Tianjian Laboratory of Advanced Biomedical Sciences State Key Laboratory of Antiviral Drugs Zhengzhou University  Zhengzhou Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6812-6342","authenticated-orcid":false,"given":"Kaixiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Key Laboratory of Advanced Drug Preparation Technologies Ministry of Education China Pingyuan Laboratory Tianjian Laboratory of Advanced Biomedical Sciences State Key Laboratory of Antiviral Drugs Zhengzhou University  Zhengzhou Henan China"},{"name":"State Key Laboratory of Metabolic Dysregulation &amp; Prevention and Treatment of Esophageal Cancer Zhengzhou University  Zhengzhou Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,3]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2021.03.016"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010021\u201000824\u20109"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41556\u2010022\u201000933\u20109"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms13608"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2016\u20100202\u2010RA"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.5c01212"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010023\u201036137\u20104"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2011.08.002"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1385"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkad953"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1258096"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.12.001"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201045163\u20109"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature24049"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41589\u2010024\u201001593\u20106"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature14592"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587\u2010025\u201002887\u20103"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1515692112"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fmre.2023.04.011"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010022\u201004677\u20102"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adz4594"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adi1965"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1101\/2025.11.16.688740"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.0c02246"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.3c04116"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajps.2023.100854"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010023\u201043552\u20100"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.06.050"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abn7382"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.4c04067"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature19802"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aam9321"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.02.011"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2022.04.021"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41569\u2010019\u20100218\u2010x"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9CS00438F"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2019.10.024"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202301766"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41588\u2010024\u201002070\u20105"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.01.017"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/dji055"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010019\u20100038\u20109"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoncol.2014.257"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2017.281295"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008\u20105472.CAN\u201016\u20101921"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551\u2010022\u201000889\u2010z"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010022\u201030862\u2010y"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202304298"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2025.105564"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1158\/1538\u20107445.AM2025\u20106961"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/mc.22342"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.7150\/jca.54574"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1002\/cac2.12327"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2018.05.042"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.4c02354"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaf886"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1070919"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1253"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.12.001"}],"container-title":["Angewandte Chemie International Edition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/anie.7088080","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/anie.7088080","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/anie.7088080","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T13:37:44Z","timestamp":1785245864000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/anie.7088080"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,3]]},"references-count":59,"journal-issue":{"issue":"31","published-print":{"date-parts":[[2026,7,27]]}},"alternative-id":["10.1002\/anie.7088080"],"URL":"https:\/\/doi.org\/10.1002\/anie.7088080","archive":["Portico"],"relation":{},"ISSN":["1433-7851","1521-3773"],"issn-type":[{"value":"1433-7851","type":"print"},{"value":"1521-3773","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,3]]},"assertion":[{"value":"2025-12-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e7088080"}}