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Conventional approaches for the analysis of protein and NA typically rely on separate workflows and instruments, limiting the reliability of point\u2010of\u2010care testing. Here, we present a digital microfluidics\u2010based dual\u2010mode molecular assay for simultaneous detection of both protein and NA from the same microscale sample. The protein is captured by a gold nanoparticle\u2013antibody conjugate, while NA is enriched by magnetic beads. Magnetic manipulation separates the protein\u2010containing fraction from NA, enabling separate analysis of the two molecular classes within an automated workflow. By integrating the analysis of protein and NA, the platform enables more accurate analysis than either modality alone. In 56 clinical samples, the combined analysis of hepatitis B surface antigen and DNA reduces the false\u2010negative rate to 1.79%, compared with 12.5% for HBsAg alone. In food safety assays, simultaneous detection of\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    \u2010associated toxins and PMA\u2010treated bacterial NA provides information not only on protein\u2010based toxins, but also the presence and viability of bacteria within milk. This automated DMF\u2010based dual\u2010mode molecular assay provides a highly integrated strategy for multimodal POCT and public health risk assessment.\n                  <\/jats:p>","DOI":"10.1002\/anie.7437306","type":"journal-article","created":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T04:05:26Z","timestamp":1792037126000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated Antigen\u2013DNA Co\u2010Detection Using Digital Microfluidics for Improving Diagnostic Accuracy in POCT Analysis"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4220-1268","authenticated-orcid":false,"given":"Fengli","family":"Chai","sequence":"first","affiliation":[{"name":"Shenzhen Key Laboratory of Smart Healthcare Engineering, Guangdong Provincial Key Laboratory of Advanced Biomaterials, Department of Biomedical Engineering Southern University of Science and Technology  Shenzhen Guangdong P. 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9647-5101","authenticated-orcid":false,"given":"Jiangjiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory of Smart Healthcare Engineering, Guangdong Provincial Key Laboratory of Advanced Biomaterials, Department of Biomedical Engineering Southern University of Science and Technology  Shenzhen Guangdong P. R. China"},{"name":"School of Life Science Beijing Institute of Technology  Beijing P. R. China"},{"name":"Zhengzhou Research Institute Beijing Institute of Technology  Zhengzhou P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Li","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory of Smart Healthcare Engineering, Guangdong Provincial Key Laboratory of Advanced Biomaterials, Department of Biomedical Engineering Southern University of Science and Technology  Shenzhen Guangdong P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4282-5759","authenticated-orcid":false,"given":"Jianzhao","family":"Zhai","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Clinical Laboratory Medicine Research Center, West China Hospital Sichuan University, Sichuan Clinical Research Center for Laboratory Medicine  Sichuan P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8077-3908","authenticated-orcid":false,"given":"Binwu","family":"Ying","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Clinical Laboratory Medicine Research Center, West China Hospital Sichuan University, Sichuan Clinical Research Center for Laboratory Medicine  Sichuan P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1004-021X","authenticated-orcid":false,"given":"Shuailong","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Mechatronical Engineering Beijing Institute of Technology  Beijing P. R. China"},{"name":"Zhengzhou Research Institute Beijing Institute of Technology  Zhengzhou P. R. China"},{"name":"State Key Laboratory of Chips and Systems for Advanced Light Field Display, School of Integrated Circuits and Electronics Beijing Institute of Technology  Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5008-4703","authenticated-orcid":false,"given":"Xingyu","family":"Jiang","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory of Smart Healthcare Engineering, Guangdong Provincial Key Laboratory of Advanced Biomaterials, Department of Biomedical Engineering Southern University of Science and Technology  Shenzhen Guangdong P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,14]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.2c10143"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.1c02311"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.2c00337"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201801815"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551\u2010022\u201000919\u2010w"},{"key":"e_1_2_8_7_1","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.abc1204","article-title":"High\u2010Throughput Single\u2010EV Liquid Biopsy: Rapid, Simultaneous, and Multiplexed Detection of Nucleic Acids, Proteins, and Their Combinations","volume":"6","author":"Zhou J.","year":"2020","journal-title":"Science 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Comprehensive Review on the Detection of Staphylococcus aureus Enterotoxins in Food Samples","volume":"23","author":"Li Q.","year":"2024","journal-title":"Comprehensive Review in Food Science and Food Safety"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.0c02378"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.1c08675"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.3c00533"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.202511107"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0SC02552F"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2021.131283"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abd6454"},{"key":"e_1_2_8_26_1","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2410164121","article-title":"DNA\u2010DISK: Automated End\u2010to\u2010End Data Storage via Enzymatic 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