{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T15:39:17Z","timestamp":1789486757117,"version":"build-2803163510"},"reference-count":53,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Angew Chem Int Ed"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Deuterium (\n                    <jats:sup>2<\/jats:sup>\n                    H) offers unique advantages as a nuclear magnetic resonance (NMR) reporter owing to its negligible endogenous biological background and ability to provide chemically distinguishable resonances. In the present study, we propose a chemical\u2010shift encoding concept in which reactions converting the deuterium\u2010bearing carbon from an sp\n                    <jats:sup>2<\/jats:sup>\n                    \u2010 to an sp\n                    <jats:sup>3<\/jats:sup>\n                    \u2010hybridized state produce pronounced and predictable\n                    <jats:sup>2<\/jats:sup>\n                    H NMR chemical\u2010shift changes, enabling distinct reaction outcomes to be encoded as spectroscopically resolvable signatures. Here we introduce deuterated \u201cmulti\u2010color\u201d probes (DMCPs), a molecular platform coupling reaction\u2010based molecular recognition with\n                    <jats:sup>2<\/jats:sup>\n                    H NMR spectroscopy to simultaneously map distinct intracellular redox pathways. DMCPs encode thiol conjugation and enzymatic reduction as spectrally separable\n                    <jats:sup>2<\/jats:sup>\n                    H NMR signatures, allowing multiplexed detection of glutathione reactivity and NAD(P)H\u2010driven reductive activity within a single acquisition. Applied in living glioblastoma cells, DMCPs reveal parallel glutathione conjugation and NAD(P)H\u2010dependent reduction, with the corresponding reaction\u2010derived signals changing upon glutathione depletion. This method further captures pharmacologically induced redox perturbations, providing a mechanistic framework for evaluating redox\u2010targeting strategies and rationalizing resistance mechanisms in early\u2010stage drug discovery.\n                  <\/jats:p>","DOI":"10.1002\/anie.7813699","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T13:17:12Z","timestamp":1789478232000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Deuterated Multi\u2010Color Probes for Reaction\u2010Resolved Mapping of Intracellular Redox Pathways With Magnetic Resonance"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4138-5205","authenticated-orcid":false,"given":"Guoshu","family":"Xie","sequence":"first","affiliation":[{"name":"Advanced Imaging Research Center University of Texas Southwestern Medical Center  Dallas Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-1151-8026","authenticated-orcid":false,"given":"Fatemeh","family":"Khashami","sequence":"additional","affiliation":[{"name":"Advanced Imaging Research Center University of Texas Southwestern Medical Center  Dallas Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8440-455X","authenticated-orcid":false,"given":"Quy Son","family":"Luu","sequence":"additional","affiliation":[{"name":"Advanced Imaging Research Center University of Texas Southwestern Medical Center  Dallas Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2129-5111","authenticated-orcid":false,"given":"Sara","family":"Chirayil","sequence":"additional","affiliation":[{"name":"Advanced Imaging Research Center University of Texas Southwestern Medical Center  Dallas Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-9977-9997","authenticated-orcid":false,"given":"Gabriel","family":"Rocha","sequence":"additional","affiliation":[{"name":"Advanced Imaging Research Center University of Texas Southwestern Medical Center  Dallas Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3354-6141","authenticated-orcid":false,"given":"Stefan","family":"Gl\u00f6ggler","sequence":"additional","affiliation":[{"name":"Advanced Imaging Research Center University of Texas Southwestern Medical Center  Dallas Texas USA"},{"name":"Department of Biomedical Engineering University of Texas Southwestern Medical Center  Dallas Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,15]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.macromol.0c02284"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573-023-00703-8"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.8b01808"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.1c00795"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aat7314"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.29379"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.4c03036"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.5c22737"},{"key":"e_1_2_8_10_1","first-page":"39","article-title":"Metabolic Imaging With Deuterium Labeled Substrates","volume":"134","author":"Low C. M. J.","year":"2023","journal-title":"Progress in Nuclear Magnetic Resonance Spectroscopy"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adr0568"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adm8600"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.201463111"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.5309"},{"key":"e_1_2_8_15_1","first-page":"571","article-title":"Deuterium Metabolic Imaging of the Brain Using 2\u2010Deoxy\u20102\u2010[2H2]\u2010d\u2010Glucose: A Non\u2010Ionizing [18F]FDG Alternative","volume":"5","author":"Gao X.","year":"2025","journal-title":"Journal of the American Chemical Society"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.4c09572"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchem.1500"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.accounts.9b00302"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.accounts.9b00314"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2016.6925"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010020\u201000326\u20100"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2020.06.001"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41565\u2010019\u20100499\u20106"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms1722"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.1142"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2018.11.002"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2020.101800"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.602664"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1158\/1541\u20107786.MCR\u201016\u20100028"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2015.3712"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M117.814392"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-017-2484-4"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2016.00156"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2013.05.004"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42255\u2010022\u201000591\u2010z"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms1506"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201804309"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.3c00683"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6CC07111B"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2022.102502"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chempr.2025.102868"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.8b00202"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010025\u201059105\u20106"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41929\u2010020\u20100488\u2010z"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.6b00788"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscentsci.3c00385"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.5c04351"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1021\/mp050096"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinorgbio.2011.01.003"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm101356p"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010020\u201020067\u20106"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M704085200"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201909803"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2022.119284"}],"container-title":["Angewandte Chemie International Edition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/anie.7813699","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/anie.7813699","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/anie.7813699","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T13:17:13Z","timestamp":1789478233000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/anie.7813699"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,15]]},"references-count":53,"alternative-id":["10.1002\/anie.7813699"],"URL":"https:\/\/doi.org\/10.1002\/anie.7813699","archive":["Portico"],"relation":{},"ISSN":["1433-7851","1521-3773"],"issn-type":[{"value":"1433-7851","type":"print"},{"value":"1521-3773","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,15]]},"assertion":[{"value":"2026-06-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e7813699"}}