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Its clinical translation, however, relies on access to mitochondria that are both of high purity and metabolically active, requirements that current isolation techniques struggle to meet. Conventional differential centrifugation (DC) method yields heterogeneous and low\u2010activity mitochondria, whereas magnetic bead (MB)\u2010based immuno\u2010isolation leaves non\u2010biodegradable beads permanently attached. Herein, we present a Light\u2010Activated Mitochondrial Isolation (\n                    <jats:bold>LAMI<\/jats:bold>\n                    ) platform comprising programmable mitochondria\u2010targeting MBs and a photo\u2010responsive release mechanism for the selective, efficient, and non\u2010destructive extraction of high\u2010quality mitochondria.\n                    <jats:bold>LAMI<\/jats:bold>\n                    employs magnetic nanoparticles decorated with a branched, modular probe architecture that supports systematic variation in mitochondria\u2010targeting ligand type, ligand density, and optical tracking elements. Incorporation of a photo\u2010cleavable linker allows on\u2010demand, mild, and reagent\u2010free release of captured mitochondria. Compared with DC method,\n                    <jats:bold>LAMI<\/jats:bold>\n                    produces mitochondria with markedly improved purity, structural integrity, and functionality. In an ischemia\u2010reperfusion injury (IRI) model,\n                    <jats:bold>LAMI<\/jats:bold>\n                    \u2010isolated mitochondria\u2010based AMT exhibits superior therapeutic performance. Together,\n                    <jats:bold>LAMI<\/jats:bold>\n                    provides a non\u2010destructive, efficient, and versatile mitochondrial isolation strategy that overcomes long\u2010standing limitations of current methods, offering a robust platform to advance AMT and its future biomedical applications.\n                  <\/jats:p>","DOI":"10.1002\/anie.7935890","type":"journal-article","created":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T09:28:11Z","timestamp":1779960491000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Light\u2010Activated Isolation of High\u2010Quality Mitochondria for Therapeutic Transplantation"],"prefix":"10.1002","volume":"65","author":[{"given":"Hui","family":"Liu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Flexible Electronics (LOFE) &amp; Institute of Flexible Electronics (IFE) Northwestern Polytechnical University  Xi'an 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