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However, the throughput is limited by the time\u2010consuming nanoLC\u2013MS analysis of numerous microdissected slices. Herein, to boost the throughput of spatial proteomics, an ordered colloidal crystal column was developed for fast nanoLC\u2013MS analysis of microdissected slices with low\u2010input amounts, down to single\u2010cell resolution. Contributed by a highly ordered arrangement of 800\u00a0nm colloidal particles, the column efficiency reached 2 560 000 plates\u00b7m\n                    <jats:sup>\u22121<\/jats:sup>\n                    , 10\u2010fold higher than that of commonly used sub\u20102\u2010\u00b5m particle packed columns, enabling robust and rapid peptide separation. With such a column, high\u2010throughput nanoLC\u2013MS analysis was achieved, as demonstrated by the identification of 5942 and 4388 proteins from 250 pg HeLa digests using 5\u2010 and 2\u2010min gradients, respectively. More notably, the column exhibited exceptional performance in single\u2010cell spatial proteomics, enabling the identification of up to 2304 proteins from a single hepatocyte slice within only a 5\u2010min gradient. Even under an ultrarapid 2\u2010min gradient, up to 1292 proteins were identified from single\u2010cell slices, which is 16 times faster than conventional methods. All these results demonstrated great promise of the colloidal crystal column for high\u2010throughput spatial proteomics with single\u2010cell resolution.\n                  <\/jats:p>","DOI":"10.1002\/anie.8045649","type":"journal-article","created":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T06:17:44Z","timestamp":1777357064000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["High\u2010Throughput Single\u2010Cell\u2010Resolved Spatial Proteomics Enabled by an Ordered Colloidal Crystal Column"],"prefix":"10.1002","volume":"65","author":[{"given":"Haofei","family":"Sun","sequence":"first","affiliation":[{"name":"State Key Laboratory of Medical Proteomics, National Chromatographic Research &amp; Analysis Center, Dalian Institute of Chemical Physics Chinese Academy of Sciences  Dalian China"},{"name":"University of Chinese Academy of Sciences  Beijing 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