{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T16:57:19Z","timestamp":1790528239301,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:00:00Z","timestamp":1790467200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:00:00Z","timestamp":1790467200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22474148"],"award-info":[{"award-number":["22474148"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22407127"],"award-info":[{"award-number":["22407127"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Angew Chem Int Ed"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Serotonin is a key neurotransmitter, and aptamer\u2010based tools using the 44\u2010nt Apt44 have been successfully developed for its in vitro and in vivo detection. Nevertheless, the structural basis of recognition by this aptamer remains unclear. Here, we report high\u2010resolution NMR structures of Apt38, a 6\u2010nt truncated variant in the third loop of Apt44, in free and serotonin\u2010bound states. Both structures reveal a two\u2010layered antiparallel chair\u2010type G\u2010quadruplex core with three edgewise loops and a terminal duplex, forming a G\u2010quadruplex\u2013duplex hybrid structure. Serotonin binds at the G\u2010quadruplex\u2013duplex junction by stacking directly onto the G\u2010tetrad plane without contacting the duplex, representing a newly identified binding mode at this hotspot junction. This binding is stabilized by stacking, electrostatic attraction, hydrogen bonding, and hydrophobic contacts. Apt38 is preorganized for binding, whereas the longer third loop of Apt44 introduces conformational dynamics into the G\u2010quadruplex scaffold, which enables a pronounced binding\u2010triggered conformational switch in PBS buffer, explaining its sensing mechanism. Our work reveals the recognition and sensing mechanism of the serotonin aptamer and provides a framework for aptamer design in serotonin biosensing.<\/jats:p>","DOI":"10.1002\/anie.8380078","type":"journal-article","created":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T16:41:39Z","timestamp":1790527299000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Recognition Mechanism of Serotonin by a G\u2010Quadruplex\u2013Duplex Hybrid Aptamer"],"prefix":"10.1002","author":[{"given":"Guohua","family":"Xu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Magnetic Resonance Spectroscopy and Imaging National Center for Magnetic Resonance in Wuhan Innovation Academy for Precision Measurement Science and Technology Chinese Academy of Sciences  Wuhan P. R. China"},{"name":"University of Chinese Academy of Sciences  Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Magnetic Resonance Spectroscopy and Imaging National Center for Magnetic Resonance in Wuhan Innovation Academy for Precision Measurement Science and Technology Chinese Academy of Sciences  Wuhan P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meiting","family":"Kang","sequence":"additional","affiliation":[{"name":"University of Chinese Academy of Sciences  Beijing P. R. China"},{"name":"State Key Laboratory of Environmental Chemistry and Toxicology Research Center for Eco\u2010Environmental Sciences Chinese Academy of Sciences  Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiawen","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Magnetic Resonance Spectroscopy and Imaging National Center for Magnetic Resonance in Wuhan Innovation Academy for Precision Measurement Science and Technology Chinese Academy of Sciences  Wuhan P. R. China"},{"name":"University of Chinese Academy of Sciences  Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junhan","family":"Wei","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Magnetic Resonance Spectroscopy and Imaging National Center for Magnetic Resonance in Wuhan Innovation Academy for Precision Measurement Science and Technology Chinese Academy of Sciences  Wuhan P. R. China"},{"name":"University of Chinese Academy of Sciences  Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3448-8756","authenticated-orcid":false,"given":"Qiang","family":"Zhao","sequence":"additional","affiliation":[{"name":"University of Chinese Academy of Sciences  Beijing P. R. China"},{"name":"State Key Laboratory of Environmental Chemistry and Toxicology Research Center for Eco\u2010Environmental Sciences Chinese Academy of Sciences  Beijing P. R. China"},{"name":"School of Environment Hangzhou Institute for Advanced Study UCAS  Hangzhou P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maili","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Magnetic Resonance Spectroscopy and Imaging National Center for Magnetic Resonance in Wuhan Innovation Academy for Precision Measurement Science and Technology Chinese Academy of Sciences  Wuhan P. R. China"},{"name":"University of Chinese Academy of Sciences  Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5798-1722","authenticated-orcid":false,"given":"Conggang","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Magnetic Resonance Spectroscopy and Imaging National Center for Magnetic Resonance in Wuhan Innovation Academy for Precision Measurement Science and Technology Chinese Academy of Sciences  Wuhan P. R. China"},{"name":"University of Chinese Academy of Sciences  Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,27]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010022\u201004615\u20102"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41557\u2010024\u201001488\u2010y"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abl6285"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.adp3897"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010021\u201003956\u20108"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010024\u201008412\u2010x"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010018\u20100416\u20104"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature19318"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010025\u201008809\u20102"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41583\u2010025\u201001003\u20103"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2200121"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/346818a0"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.0c01140"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.4c04993"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abn9859"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202008663"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.adv6127"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202318665"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41557\u2010023\u201001207\u2010z"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2025.115680"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aao6750"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c03764"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202519635"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c05038"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41380\u2010021\u201001066\u20105"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abj7422"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/S40580\u2010022\u201000325\u20107"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.2c00035"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssensors.2c01909"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2108118120"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.2c03344"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5c05203"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202504497"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202501701"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41565\u2010023\u201001591\u20100"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.3c05377"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1039\/d3cc04334g"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.202005026"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.202003540"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.202103718"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.2c04775"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202106256"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202309891"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.2c00478"}],"container-title":["Angewandte Chemie International Edition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/anie.8380078","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/anie.8380078","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/anie.8380078","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T16:41:40Z","timestamp":1790527300000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/anie.8380078"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,27]]},"references-count":44,"alternative-id":["10.1002\/anie.8380078"],"URL":"https:\/\/doi.org\/10.1002\/anie.8380078","archive":["Portico"],"relation":{},"ISSN":["1433-7851","1521-3773"],"issn-type":[{"value":"1433-7851","type":"print"},{"value":"1521-3773","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,27]]},"assertion":[{"value":"2026-07-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e8380078"}}