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Here, we report streamlined access to\n                    <jats:italic>Se<\/jats:italic>\n                    \u2010linked Tn glycopeptides which, together with their\n                    <jats:italic>O<\/jats:italic>\n                    \u2010 and\n                    <jats:italic>S<\/jats:italic>\n                    \u2010linked counterparts, enable systematic atom\u2010level editing at the glycosidic linkage of MUC1 glycopeptide antigens. Surface plasmon resonance established a clear hierarchy of antigen recognition that was rationalized by molecular dynamics simulations and independently validated by STD\u2010NMR epitope mapping, revealing that O\u2192S\u2192Se substitution subtly remodels glycopeptide presentation while preserving the overall 5E5 recognition epitope. Translation of these structurally defined antigens into CRM\n                    <jats:sub>197<\/jats:sub>\n                    glycoconjugate vaccines with comparable antigen loading showed that neither antigen\u2010binding affinity nor antibody levels alone predict therapeutic efficacy. Instead, the results demonstrate that minimal atom\u2010level editing propagates from molecular recognition to biological function through a nonlinear structure\u2013function relationship, highlighting that therapeutic efficacy cannot be inferred directly from antigen affinity alone.\n                  <\/jats:p>","DOI":"10.1002\/anie.8540595","type":"journal-article","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T04:50:58Z","timestamp":1790052658000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Single\u2010Atom O\/S\/Se Substitution at the Glycosidic Linkage in Glycopeptide Cancer Vaccines Reveals Divergent Performance"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3531-6676","authenticated-orcid":false,"given":"Carmen","family":"Bret\u00f3n","sequence":"first","affiliation":[{"name":"Departamento de Qu\u00edmica Instituto de Investigaci\u00f3n en Qu\u00edmica de La Universidad de La Rioja (IQUR). 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