{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T18:55:52Z","timestamp":1791226552428,"version":"4.1.0"},"reference-count":55,"publisher":"Wiley","issue":"41","license":[{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Angew Chem Int Ed"],"published-print":{"date-parts":[[2026,10,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Many bacteria, but not mammalian cells, can directly import and metabolize extracellular gluconate via gluconate permease (GntP) and kinase (GntK). We hypothesized that fluorine\u201018 labeled gluconic acid ([\n                    <jats:sup>18<\/jats:sup>\n                    F]FGA) could leverage unique bacterial gluconate metabolism for pathogen\u2010specific PET imaging. Here, we report [\n                    <jats:sup>18<\/jats:sup>\n                    F]FGA as a new PET tracer targeting bacterial gluconate metabolism. The chemoenzymatic radiosynthesis of [\n                    <jats:sup>18<\/jats:sup>\n                    F]FGA was simple, only requiring readily available [\n                    <jats:sup>18<\/jats:sup>\n                    F]FDG and glucose oxidase. [\n                    <jats:sup>18<\/jats:sup>\n                    F]FGA showed broad detection sensitivity across multiple bacterial species, including multidrug\u2010resistant clinical isolates.\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    transposon mutant studies showed substantial loss of [\n                    <jats:sup>18<\/jats:sup>\n                    F]FGA uptake in GntP and GntK mutants, supporting high specificity for bacterial gluconate metabolism. [\n                    <jats:sup>18<\/jats:sup>\n                    F]FGA PET selectively highlighted live bacterial infection, with high target\u2010to\u2010nontarget ratios in\n                    <jats:italic>S. aureus<\/jats:italic>\n                    (36\u2010fold) and\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    (30\u2010fold) in a murine model of myositis. Unlike [\n                    <jats:sup>18<\/jats:sup>\n                    F]FDG, no appreciable uptake of [\n                    <jats:sup>18<\/jats:sup>\n                    F]FGA was detected at sites of sterile inflammation. In a\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    pneumonia model, [\n                    <jats:sup>18<\/jats:sup>\n                    F]FGA showed significantly higher accumulation in the infected lung (5.2 \u00b1 0.8%IA\/cc) than in the uninfected lung (0.1 \u00b1 0.0%IA\/cc), further supporting its potential for imaging challenging clinical infections. Taken together, [\n                    <jats:sup>18<\/jats:sup>\n                    F]FGA PET may be a useful tool for pathogen\u2010targeted imaging in clinical practice.\n                  <\/jats:p>","DOI":"10.1002\/anie.8729932","type":"journal-article","created":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T13:20:31Z","timestamp":1786454431000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Chemoenzymatic Radiosynthesis of a Gluconate Transporter\u2010Targeted In Vivo Bacterial Sensor From Clinical [\n                    <sup>18<\/sup>\n                    F]FDG"],"prefix":"10.1002","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8578-9968","authenticated-orcid":false,"given":"Sang Hee","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Radiology and Biomedical Imaging University of California  San Francisco California 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