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However, characterizing oxidation\u2010state heterogeneity of individual proteins or peptides remains challenging, as conventional ensemble methods average over coexisting species. Here, we report a nanopore strategy based on a confined hydrogen\u2010bond network to decode oxidation states within individual native peptides. By engineering a constricted recognition region enriched with hydrogen\u2010bond interactions, the changes induced by diverse oxidation states were specifically recognized. This enables discrimination of closely related oxidative modifications, including proline hydroxylation, methionine oxidation, and tryptophan oxidation, even at ultra\u2010low abundance. We show that the reversible and regulated oxidation state could be clearly distinguished from irreversible and damaged oxidation with the same mass without separation or purification. Furthermore, we quantified heterogeneous oxidation distributions generated under controlled oxidative conditions. This approach provides a molecular\u2010level strategy for characterizing oxidation\u2010state heterogeneity in individual peptides, with potential applications in studying oxidative modifications associated with aging and disease in future biological investigations.<\/jats:p>","DOI":"10.1002\/anie.9376050","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T03:52:13Z","timestamp":1788925933000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Decoding Oxidation State Landscapes Within Individual Native Peptides by Nanopore"],"prefix":"10.1002","volume":"65","author":[{"given":"Jun\u2010Ge","family":"Li","sequence":"first","affiliation":[{"name":"Molecular Sensing and Imaging Center, School of Chemistry Nanjing University  Nanjing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4894-5973","authenticated-orcid":false,"given":"Jie","family":"Jiang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Precision and Intelligent Chemistry University of Science and Technology of China  Hefei P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Gao","sequence":"additional","affiliation":[{"name":"Molecular Sensing and Imaging Center, School of Chemistry Nanjing University  Nanjing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Zhou","sequence":"additional","affiliation":[{"name":"Molecular Sensing and Imaging Center, School of Chemistry Nanjing University  Nanjing P. R. 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