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Four synthetic protochelin (PC) analogues, KK\u2010COOH, KO\u2010NH\n                    <jats:sub>2<\/jats:sub>\n                    , KO\u2010COOH, and OK\u2010COOH were constructed. Growth recovery, radioactive uptake, and covalent photocrosslinker studies in\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    identified CirA and Fiu as the dominant outer membrane transport proteins capable of binding KO\u2010NH\n                    <jats:sub>2<\/jats:sub>\n                    \u2010linked, bifunctional constructs. Computational studies further supported that the structural framework of PC can be effectively mimicked by KO or OK peptides. Thermodynamic stability studies revealed an atypical inversion of thermodynamic stability trends, with logK\n                    <jats:sub>GaL<\/jats:sub>\n                    of 39.9 and a logK\n                    <jats:sub>FeL<\/jats:sub>\n                    of 36, demonstrating improved stability with Ga\n                    <jats:sup>3+<\/jats:sup>\n                    . Studies in na\u00efve mice with\n                    <jats:sup>68<\/jats:sup>\n                    Ga\u2010labeled derivatives identify the lead [\n                    <jats:sup>68<\/jats:sup>\n                    Ga][Ga(KO\u2010NH\n                    <jats:sub>2<\/jats:sub>\n                    )]\n                    <jats:sup>3\u2212<\/jats:sup>\n                    , which remained intact in vivo and demonstrated selective, enhanced uptake in an\n                    <jats:italic>E. coli<\/jats:italic>\n                    soft tissue model of infection (1.28 % ID\/g, with a 3:1 ratio of infected to inflamed\/normal muscle tissue). Linkage of KO\u2010NH\n                    <jats:sub>2<\/jats:sub>\n                    to the antibiotic ampicillin produces an efficacious antibiotic, which supersedes the potency of the parent antibiotic ampicillin 4\u2010fold (MIC\n                    <jats:sub>98<\/jats:sub>\n                    = 1.9\u00a0\u00b5M) effectively treats a soft tissue infection of ampicillin\u2010resistant\n                    <jats:italic>E. coli<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1002\/anie.9696366","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T08:57:51Z","timestamp":1788512271000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Rational Design of a Synthetic Galliphore for the Diagnostic Nuclear Imaging and Treatment of Bacterial 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Madison  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6418-6021","authenticated-orcid":false,"given":"Hannah","family":"Carlisle","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Wisconsin Madison  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4186-6586","authenticated-orcid":false,"given":"Eszter","family":"Boros","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Wisconsin Madison  Madison Wisconsin 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