{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T17:02:43Z","timestamp":1775840563826,"version":"3.50.1"},"reference-count":14,"publisher":"Wiley","issue":"11-12","license":[{"start":{"date-parts":[[2024,11,2]],"date-time":"2024-11-02T00:00:00Z","timestamp":1730505600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Analytical Science Advances"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Testosterone is a critical hormone involved in regulating various physiological processes in both men and women. Accurate testosterone measurement is essential for diagnosing endocrine disorders such as hypogonadism and polycystic ovary syndrome and for routine testing. Traditionally, testosterone levels are measured using serum or plasma samples, which present challenges in sample collection, storage, and transport, particularly in resource\u2010limited settings. Dried blood spot (DBS) sampling has emerged as an effective alternative for hormone analysis, offering significant advantages in terms of sample stability, ease of collection, and simplified logistics. This study aimed to validate a DBS\u2010based testosterone assay using liquid chromatography\u2010tandem mass spectrometry (LC\u2010MS\/MS) to ensure accuracy and precision comparable to conventional serum\u2010based methods. Drops of whole blood samples were collected from adult volunteers using a single\u2010use safety lancet for finger pricks, with blood applied onto DBS cards (PerkinElmer 226 Spot Saver RUO card) for further analysis. The testosterone was extracted from DBS using a liquid\u2010liquid method and analyzed with LC\u2010MS\/MS. The assay demonstrated excellent linearity across a wide concentration range (0.1\u2013100\u00a0ng\/mL) with a correlation coefficient (r<jats:sup>2<\/jats:sup>) of 0.999 and achieved a lower limit of detection of 0.058\u00a0ng\/mL and a lower limit of quantification of 0.086\u00a0ng\/mL. The method showed high precision, with intra\u2010 and inter\u2010day coefficients of variation below 10%, and satisfactory recovery rates. Hematocrit correction and matrix effect evaluations confirmed the robustness of the assay for clinical and research applications. Additionally, the assay displayed a strong clinical correlation between testosterone levels in DBS and venous serum samples, supporting its reliability for testosterone monitoring. This validation study supports that the DBS\u2010based LC\u2010MS\/MS testosterone assay is a reliable tool for testosterone quantification for routine testing.<\/jats:p>","DOI":"10.1002\/ansa.202400035","type":"journal-article","created":{"date-parts":[[2024,11,2]],"date-time":"2024-11-02T17:39:00Z","timestamp":1730569140000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Validation of a Dried Blood Spot Assay for Testosterone Measurement Using Liquid Chromatography\u2010Tandem Mass Spectrometry"],"prefix":"10.1002","volume":"5","author":[{"given":"Yehudah","family":"Gruenstein","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology University of Miami  Coral Gables Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,11,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.maturitas.2019.04.203"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1017\/CBO9781139003353"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2007.031"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2024.106496"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2013-0228"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/0004563220937735"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0233724"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00414-012-0799-y"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.5555"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.17-0889"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202300210"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/131.5.1631S"},{"issue":"6","key":"e_1_2_7_14_1","first-page":"356","article-title":"Ultra Performance Liquid Chromatography (UPLC)","volume":"4","author":"Samatha Y.","year":"2015","journal-title":"World Journal of Pharmacy and Pharmaceutical Sciences"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11095-021-03036-6"}],"container-title":["Analytical Science Advances"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ansa.202400035","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T18:43:16Z","timestamp":1759948996000},"score":1,"resource":{"primary":{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/10.1002\/ansa.202400035"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,2]]},"references-count":14,"journal-issue":{"issue":"11-12","published-print":{"date-parts":[[2024,12]]}},"alternative-id":["10.1002\/ansa.202400035"],"URL":"https:\/\/doi.org\/10.1002\/ansa.202400035","archive":["Portico"],"relation":{},"ISSN":["2628-5452","2628-5452"],"issn-type":[{"value":"2628-5452","type":"print"},{"value":"2628-5452","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,2]]},"assertion":[{"value":"2024-08-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e202400035"}}