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We report the distinct and measurable colour changes when xylazine is exposed to the Mandelin, Marquis and Mecke presumptive test reagents. The colour changes observed with xylazine are distinct from those of drugs that give colour changes from the same presumptive tests. To overcome the subjective limitations of determining spot test results by\u2010eye, we applied image and video analyses to quantify the distinctive features of presumptive tests with xylazine and thus differentiate it from other illicit substances tested under the same conditions, including morphine, fentanyl, heroin and methamphetamine. Herein, experimental protocols utilising\n                    <jats:italic>Kineticolor<\/jats:italic>\n                    , a computer vision software, were developed to qualitatively and quantitatively study presumptive tests for xylazine detection. To the best of our knowledge, these findings represent the first presumptive test strategy towards specific, quantifiable and potentially field\u2010ready detection of xylazine.\n                  <\/jats:p>","DOI":"10.1002\/ansa.70008","type":"journal-article","created":{"date-parts":[[2025,4,3]],"date-time":"2025-04-03T20:24:40Z","timestamp":1743711880000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Presumptive Tests for Xylazine\u2014A Computer Vision Approach"],"prefix":"10.1002","volume":"6","author":[{"given":"Hui Yun","family":"Chang","sequence":"first","affiliation":[{"name":"Department of Pure and Applied Chemistry University of Strathclyde  Glasgow UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristin","family":"Donnachie","sequence":"additional","affiliation":[{"name":"Department of Pure and Applied Chemistry University of Strathclyde  Glasgow UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy J. 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