{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T03:23:45Z","timestamp":1791948225981,"version":"4.3.4"},"reference-count":45,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T00:00:00Z","timestamp":1758758400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Analytical Science Advances"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Medicinal plants are a global healthcare cornerstone, with traditional communities, particularly in Ethiopia, relying on indigenous knowledge for treating various ailments. This study aimed to identify, isolate, and elucidate the structures of chemical constituents from the chloroform and methanol extracts of\n                    <jats:italic>Syzygium guineense<\/jats:italic>\n                    root using IR, 1D, and 2D NMR spectroscopic techniques in South Ethiopia of Woliata Zone. Preliminary phytochemical screening confirmed the presence of various secondary metabolites, including alkaloids, flavonoids, terpenoids, polyphenols, saponins, tannins, glycosides, and steroids. The result indicated that the powdered root material was sequentially extracted with chloroform and methanol, yielding 5\u00a0g of chloroform extract (SGA) and 9\u00a0g of methanol extract (SGB). Subsequent chromatographic fractionation of the extracts led to the isolation of two pure compounds: SGB\u20103 (25\u00a0mg) from the methanol extract and SGA\u201024 (100\u00a0mg) from the chloroform extract. The structural elucidation of both compounds was performed using 1D and 2D NMR spectroscopy. The spectroscopic data (IR,\n                    <jats:sup>1<\/jats:sup>\n                    H NMR,\n                    <jats:sup>1<\/jats:sup>\n                    <jats:sup>3<\/jats:sup>\n                    C NMR, and DEPT) of SGB\u20103 indicated a diterpene lactone, characterized by hydroxyl, olefinic, and lactone functionalities. Partial characterization of SGA\u201024 through 1D and 2D NMR (\n                    <jats:sup>1<\/jats:sup>\n                    H NMR,\n                    <jats:sup>1<\/jats:sup>\n                    <jats:sup>3<\/jats:sup>\n                    C NMR, DEPT, HMBC, HSQC) revealed its identity as a terpenoid with an oleanane \u0394\n                    <jats:sup>1<\/jats:sup>\n                    <jats:sup>2<\/jats:sup>\n                    \u2010ene skeleton, showing close similarities to arjunolic acid. These findings contribute valuable scientific evidence to the phytochemistry of\n                    <jats:italic>Syzygium guineense<\/jats:italic>\n                    roots from South Ethiopia, supporting its traditional uses and identifying potential lead compounds for further pharmacological investigations.\n                  <\/jats:p>","DOI":"10.1002\/ansa.70047","type":"journal-article","created":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T19:34:48Z","timestamp":1758828888000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Phytochemical Investigation and Isolation of Chemical Constituents From the Root Extract of\n                    <i>Syzygium guineense<\/i>\n                    in South Ethiopia"],"prefix":"10.1002","volume":"6","author":[{"given":"Mekdes","family":"Lulu","sequence":"first","affiliation":[{"name":"College of Agriculture Department of Plant Science Wachemo University  Hossana Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,25]]},"reference":[{"key":"e_1_2_7_2_1","article-title":"Ethnobotanical Study of Traditional Medicinal Plants Used by Local Communities in Metema District, Northwestern Ethiopia","volume":"14","author":"Gebremeskel A.","year":"2023","journal-title":"Frontiers in Pharmacology"},{"key":"e_1_2_7_3_1","article-title":"Advancing Medicinal Plant Agriculture: Integrating Technology and Precision Agriculture for Sustainability","volume":"15","author":"Kaur P.","year":"2024","journal-title":"Frontiers in Plant Science"},{"key":"e_1_2_7_4_1","first-page":"135","volume-title":"Biotechnology, Multiple Omics, and Precision Breeding in Medicinal Plants","author":"Malik A.","year":"2022"},{"issue":"1","key":"e_1_2_7_5_1","first-page":"1","article-title":"Ethnopharmacological Study of Traditional Medicinal Plants Used by the People in Metema District, Northwestern Ethiopia","volume":"23","author":"Getaneh S.","year":"2023","journal-title":"BMC Complementary Medicine and Therapies"},{"key":"e_1_2_7_6_1","first-page":"1","article-title":"Ethnobotanical Study of Medicinal Plants Used for the Treatment of human Ailments in the Sodo District of East Gurage Zone Central Ethiopia","volume":"30","author":"Tessema Z. 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