{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:29:30Z","timestamp":1786976970847,"version":"build-2736575974"},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T00:00:00Z","timestamp":1763337600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002462","name":"Chungnam National University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002462","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Analytical Science Advances"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Tagetes erecta<\/jats:italic>\n                    L. is widely studied for its flower\u2010derived lutein, which is known for promoting eye health. However, its roots contain uniquely thiophenes\u2014absent from the flowers and leaves\u2014which exhibit valuable bioactivity. Our recent study has reported on patent applications related to their efficacy in modulating benign prostatic hyperplasia, underscoring their pharmaceutical and functional potential. Despite this, no optimized extraction or quality evaluation of thiophene\u2010rich\n                    <jats:italic>T. erecta<\/jats:italic>\n                    roots has been reported. This study aimed to establish a reliable extraction strategy for thiophene\u2010rich\n                    <jats:italic>T. erecta<\/jats:italic>\n                    roots and to ensure chemical consistency through validated HPLC quantification and chromatographic fingerprint similarity analysis. Three major thiophenes\u20145\u2010(4\u2010hydroxybut\u20101\u2010ynyl)\u20102,2'\u2010bithiophene (\n                    <jats:bold>1<\/jats:bold>\n                    ), 5\u2010(4\u2010acetoxybut\u20101\u2010ynyl)\u20102,2'\u2010bithiophene (\n                    <jats:bold>2<\/jats:bold>\n                    ) and 5\u2010(3\u2010buten\u20101\u2010ynyl)\u20102,2'\u2010bithiophene (\n                    <jats:bold>3<\/jats:bold>\n                    )\u2014were isolated and structurally confirmed. The extraction variables (solvent, plant part, method, sample amount and time) were optimized. Quantification was performed via validated HPLC\u2010PDA using in\u2010house purified standards (\u2265\u00a098%), and fingerprint similarity was evaluated. Ultrasonic extraction for 2\u00a0h with 5\u00a0g of root powder in 95% ethanol gave optimal yield and reproducibility. The HPLC method exhibited excellent linearity (\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sup>2\u00a0<\/jats:sup>\n                    &gt;\u00a0\u00a00.999), precision (RSD\u00a0&lt;\u00a01%) and recovery (93.20%\u2013105.24%). Fingerprint analysis of 13 common peaks revealed high similarity (0.984\u20131.000), indicating stable chemical profiles. This study provides a validated workflow for thiophene\u2010rich\n                    <jats:italic>T. erecta<\/jats:italic>\n                    roots, highlights the significance of root\u2010derived thiophenes and offers a practical basis for process development, quality control and future standardization of thiophenes and related constituents.\n                  <\/jats:p>","DOI":"10.1002\/ansa.70055","type":"journal-article","created":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T00:15:44Z","timestamp":1763424944000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Integrated Extraction Optimization and HPLC\u2010based Quality Evaluation of Thiophenes from\n                    <i>Tagetes erecta<\/i>\n                    Roots"],"prefix":"10.1002","volume":"6","author":[{"given":"Shuo","family":"Tian","sequence":"first","affiliation":[{"name":"College of Pharmacy Chungnam National University Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sainan","family":"Li","sequence":"additional","affiliation":[{"name":"College of Pharmacy Chungnam National University Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jisu","family":"Park","sequence":"additional","affiliation":[{"name":"College of Pharmacy Chungnam National University Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jong\u2010Sup","family":"Bae","sequence":"additional","affiliation":[{"name":"College of Pharmacy Kyungpook National University  Daegu Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MinKyun","family":"Na","sequence":"additional","affiliation":[{"name":"College of Pharmacy Chungnam National University Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,17]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtcme.2024.08.001"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.17795\/jjhr\u201029744"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.53517\/cmdr.2581\u20105008.412020201"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.3950"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants11040539"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.9b01306"},{"key":"e_1_2_11_8_1","unstructured":"H. J.AnandM.Na \u201cComposition for Preventing Improving or Treating Benign Prostatic Hyperplasia Comprising Marigold Root Extract \u201d Patent no. 10\u20132025\u20130055153 Korean Patent Application 2025."},{"key":"e_1_2_11_9_1","unstructured":"H. J.AnandM.Na \u201cComposition for Preventing Improving or Treating Benign Prostatic Hyperplasia Comprising Compound Isolated From Marigold \u201d Patent no. 10\u20132025\u20130055154 Korean Patent Application 2025."},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.15244\/pjoes\/110612"},{"issue":"2","key":"e_1_2_11_11_1","first-page":"10","article-title":"Caracterizacion Fisica y Tamizaje Fitoquimico de la Especie Tagetes erecta Lin","volume":"21","author":"Barrera\u2010roca L.","year":"2009","journal-title":"Revista Cubana de Qu\u00edmica"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/je050097a"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0032\u20103861(01)00404\u20109"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389557523666230206104257"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.36713\/epra13592"},{"key":"e_1_2_11_16_1","first-page":"546","article-title":"Isolation and Quantitative Analysis of Thiophenes From Tagetes Species by HPLC","volume":"79","author":"Verma V. 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