{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T15:28:16Z","timestamp":1787066896508,"version":"build-2736575974"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T00:00:00Z","timestamp":1775433600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T00:00:00Z","timestamp":1775433600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Analytical Science Advances"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The pharmaceutical market has recently witnessed the advent of a novel tetrahydrotriazene molecule belonging to the new pharmacological class \u201cGlimins\u201d, named Imeglimin (IMG). It has been approved in Japan as a safe and highly effective oral diabetic drug for type II diabetic patients. It enhances the function of \u03b2\u2010cells in the human pancreas and increases insulin sensitivity. Hence, this work was directed to provide a fast, sensitive and highly reliable bioanalytical method for its quantification in human plasma. What makes this work greatly distinctive is first: adopting an ultra\u2010performance liquid chromatography (UPLC) with protein precipitation as a fast and straightforward sample preparation protocol with the highest extraction recovery. Secondly, utilisation of the stable isotope\u2010labelled molecule, IMG\u2010d6, rather than a structurally similar analogue, to avoid the interference of any co\u2010administered drugs in human plasma. Samples were extracted with acetonitrile and then chromatographically separated using an Acquity UPLC BEH HILIC column of 1.7\u00a0\u00b5m particle size along with a mobile phase solution composed of 70:30 (v\/v) acetonitrile and 10\u00a0mM ammonium formate buffer acidified with 0.1% formic acid. Upon adjusting the flow rate to 0.3\u00a0mL\/min, IMG was eluted at 1.3 min with a total run time of only 2.0 min. Mass quantification was performed in positive electrospray ionisation operated in multiple reaction monitoring mode. IMG was quantified at\n                    <jats:italic>m\/z<\/jats:italic>\n                    of 156.05 \u2192 112.91 transition pairs while IMG\u2010d6, at\n                    <jats:italic>m\/z<\/jats:italic>\n                    162.11 \u2192 119.04. The proposed UPLC\u2010tandem mass spectrometry method was thoroughly validated as per Food and Drug Administration principles over a linearity range of 10.0\u20133000.0\u00a0ng\/mL and successfully applied for IMG quantification in human plasma samples. The scope of the work was extended to encompass real analysis of collected human blood samples, calculation of IMG pharmacokinetics parameters and conductance of a bioequivalence study between the IMG generic product versus brand one.\n                  <\/jats:p>","DOI":"10.1002\/ansa.70079","type":"journal-article","created":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T19:54:48Z","timestamp":1775505288000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A Novel Ultra\u2010Performance Liquid Chromatography\u2010Electrospray Ionisation\u2010Tandem Mass Spectrometry Method for Quantification of Imeglimin in Human Plasma: Application to a Bioequivalence Study"],"prefix":"10.1002","volume":"7","author":[{"given":"Mina","family":"Wadie","sequence":"first","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department Faculty of Pharmacy, Cairo University  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamal A.","family":"Badr","sequence":"additional","affiliation":[{"name":"Advanced Research Centre (ARC)  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed A.","family":"Hosny","sequence":"additional","affiliation":[{"name":"Research and Development (R&amp;D) Department Atco Pharma for Pharmaceutical Industries  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liliya","family":"Logoyda","sequence":"additional","affiliation":[{"name":"Pharmaceutical Chemistry Department Pharmaceutical Faculty I. Horbachevsky Ternopil National Medical University  Ternopil Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omnia M. El","family":"Sebai","sequence":"additional","affiliation":[{"name":"Advanced Research Centre (ARC)  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mamdouh R.","family":"Rezk","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department Faculty of Pharmacy, Cairo University  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,6]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11886\u2010019\u20101107\u2010y"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.2174\/1570161117666190405165911"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213\u20108587(21)00113\u20103"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dsx.2023.102710"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40268\u2010015\u20100099\u20103"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40262\u2010020\u201000948\u20101"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40265\u2010021\u201001589\u20109"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2009.10.004"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2015.05.006"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10895\u2010024\u201004020\u20100"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40268\u2010025\u201000526\u20105"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2025.128915"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00210\u2010026\u201005017\u20101"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talo.2025.100457"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/ansa.70028"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scp.2025.101923"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1039\/D5AY02026C"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2026.117217"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2023.123650"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2019.01.058"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scp.2024.101827"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13065\u2010025\u201001661\u20105"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/48.8.624"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2008.12.025"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2014.12.098"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10008\u2010018\u20104045\u20105"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216\u2010013\u20107542\u20105"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/ddr.20173"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2025.124576"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2014.12.002"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2005.06.035"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10337\u2010013\u20102477\u20108"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2014.05.005"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.1560"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.4137"},{"key":"e_1_2_11_37_1","unstructured":"Food and Drug Administration (FDA) M10 Bioanalytical Method Validation and Study Sample Analysis; 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