{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T21:05:02Z","timestamp":1784840702871,"version":"3.55.0"},"reference-count":48,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T00:00:00Z","timestamp":1784764800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T00:00:00Z","timestamp":1784764800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Analytical Science Advances"],"published-print":{"date-parts":[[2026,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Acampe papillosa<\/jats:italic>\n                    , an orchid species traditionally utilised as an herbal medicine, has yet to be extensively researched for its potential pharmacological properties. This study examined the potential for anti\u2010anxiety, sedative, anti\u2010inflammatory and antipyretic effects of methanol extract of the stem of\n                    <jats:italic>A. papillosa<\/jats:italic>\n                    (APSME) through in vivo and in silico approaches. A dose of 200 and 400\u00a0mg\/kg was administered to Swiss albino rodents. When examined in the elevated plus maze, APSME\u2010treated mice spent significantly longer periods of time in the open arms of the maze at both dosages than control animals (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.001), indicating an anxiolytic effect of APSME. The sedative effects of APSME were assessed by measuring locomotor activity using an open field test and a hole cross test, with both methods showing a significant decrease in locomotion as APSME doses increased from 200 to 400\u00a0mg. Following treatment of rodents with carrageenan\u2010induced paw oedema using APSME (400\u00a0mg\/kg), significant reductions in the thickness of the paw were observed at 4 h post\u2010administration compared to control groups (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.001), indicating strong anti\u2010inflammatory activity. Antipyretic effects of APSME were measured using a brewer's yeast\u2010induced pyrexia animal model and were accompanied by statistically significant reductions in rectal temperature at 21 h post\u2010treatment (98.04 \u00b1 0.05\u00b0F). Molecular docking and PASS prediction further support these findings by showing favourable interactions between the plant phytochemicals and relevant biological targets. Overall, the results suggest that\n                    <jats:italic>A. papillosa<\/jats:italic>\n                    could be a promising natural source for developing treatments for anxiety, inflammation, fever and related conditions.\n                  <\/jats:p>","DOI":"10.1002\/ansa.70097","type":"journal-article","created":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T20:58:32Z","timestamp":1784840312000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated Phytochemical Characterisation, Pharmacological Evaluation and Computational Investigation of\n                    <i>Acampe papillosa<\/i>\n                    Stem Extract"],"prefix":"10.1002","volume":"7","author":[{"given":"Qurratul Ain","family":"Sadia","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Faculty of Biological Sciences University of Chittagong  Chattogram Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sadia Tamanna","family":"Tamim","sequence":"additional","affiliation":[{"name":"Department of Pharmacy BGC Trust University Bangladesh  Chattogram Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faruq Mohammed","family":"Tashriq","sequence":"additional","affiliation":[{"name":"Department of Pharmacy BGC Trust University Bangladesh  Chattogram Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nasir","family":"Uddin","sequence":"additional","affiliation":[{"name":"Department of Pharmacy BGC Trust University Bangladesh  Chattogram Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatematus Zhohara Alam","family":"Hima","sequence":"additional","affiliation":[{"name":"Department of Pharmacy BGC Trust University Bangladesh  Chattogram Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulfat Sobha","family":"Surat","sequence":"additional","affiliation":[{"name":"Department of Pharmacy BGC Trust University Bangladesh  Chattogram Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mily","family":"Khastagir","sequence":"additional","affiliation":[{"name":"Department of Pharmacy BGC Trust University Bangladesh  Chattogram Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Abdul","family":"Jalil","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Biological Sciences University of Chittagong  Chattogram Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Hossain","family":"Rasel","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Biological Sciences University of Chittagong  Chattogram Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arman Ullah","family":"Rafi","sequence":"additional","affiliation":[{"name":"Department of Pharmacy BGC Trust University Bangladesh  Chattogram Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7168-3257","authenticated-orcid":false,"given":"S. M. Moazzem","family":"Hossen","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Biological Sciences University of Chittagong  Chattogram Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,23]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.2174\/1568026616666160204121039"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/da.22026"},{"key":"e_1_2_12_4_1","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/j.jad.2014.06.021","article-title":"Risk Factors for the Onset of Panic and Generalised Anxiety Disorders in the General Adult Population: A Systematic Review of Cohort Studies","volume":"168","author":"Moreno\u2010Peral P.","year":"2014","journal-title":"Journal of Affective Disorders"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2011\u20102947"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMcp1502514"},{"issue":"4","key":"e_1_2_12_7_1","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1097\/00005082-200407000-00003","article-title":"Sleep Difficulties, Daytime Sleepiness, and Health\u2010related Quality of Life in Patients With Chronic Heart Failure","volume":"19","author":"Brostr\u00f6m A.","year":"2004","journal-title":"Journal of Cardiovascular Nursing"},{"issue":"2","key":"e_1_2_12_8_1","first-page":"210","article-title":"The Diagnosis and Management of Insomnia in Clinical Practice: A Practical Evidence\u2010based Approach","volume":"162","author":"Holbrook A. 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