{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T04:00:41Z","timestamp":1790308841036,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:00:00Z","timestamp":1790208000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:00:00Z","timestamp":1790208000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Analytical Science Advances"],"published-print":{"date-parts":[[2026,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Characterising chemical and biological differences among different tissues of medicinal plants is important for understanding their tissue\u2010specific characteristics and medicinal efficacy. The root xylem (RX) and root bark (RB) of\n                    <jats:italic>Tripterygium wilfordii<\/jats:italic>\n                    have historically been used differently; however, the basis underlying their differential utilisation remains insufficiently characterised. In this study, complementary analytical measurements were integrated to compare the chemical and biological differences between the two root tissues across multiple dimensions. Ion fingerprinting revealed substantial overlap in the detected chemical features, with 81.09% of the ion features in the RX also detectable in the RB. Despite this high proportion of shared constituents, multivariate statistical analysis clearly separated the RX and RB, indicating significant differences in their overall ion abundance patterns. Mass spectrometry imaging further resolved tissue\u2010specific distribution patterns, showing that alkaloids were mainly localised in the phloem, triterpenoids were enriched in the periderm, whereas diterpenoids exhibited broader distributions across the root tissues. Targeted quantitative analysis provided direct evidence for the differential abundance of metabolites, showing that multiple bioactive constituents, including triptolide, triptonide and celastrol, were present at considerably higher levels in the RB than in the RX. Biological evaluation further demonstrated that, compared with the RX, the RB exhibited stronger inhibition of lipopolysaccharide\u2010induced nitric oxide production, albeit with higher cytotoxicity. Taken together, the complementary measurements confirmed that, although the RX and RB share a large number of chemical features, they differ markedly in overall metabolite abundance and spatial distribution, ultimately exhibiting distinct biological responses. This study established a multidimensional analytical framework for resolving tissue\u2010specific chemical variation in complex medicinal plant materials and demonstrates its utility for integrating chemical profiling, spatial mapping, targeted quantification and biological evaluation.\n                  <\/jats:p>","DOI":"10.1002\/ansa.70112","type":"journal-article","created":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T03:28:31Z","timestamp":1790306911000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Multimodal Analytical Platform Coupled With Biological Assays Unravels Tissue\u2010Specific Chemical Profiles, Spatial Metabolite Distributions and Bioactivities of Root Xylem and Root Bark of\n                    <i>Tripterygium wilfordii<\/i>"],"prefix":"10.1002","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0554-3755","authenticated-orcid":false,"given":"Zhong Mou","family":"Zhang","sequence":"first","affiliation":[{"name":"National Institutes for Drug Control  Beijing China"},{"name":"School of Traditional Chinese Medicine Beijing University of Chinese Medicine  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai Ling","family":"Xu","sequence":"additional","affiliation":[{"name":"Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Institutes for Drug Control  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong Qiang","family":"Lin","sequence":"additional","affiliation":[{"name":"National Institutes for Drug Control  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7598-0723","authenticated-orcid":false,"given":"Feng","family":"Wei","sequence":"additional","affiliation":[{"name":"National Institutes for Drug Control  Beijing China"},{"name":"School of Traditional Chinese Medicine Beijing University of Chinese Medicine  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya","family":"Dan Wang","sequence":"additional","affiliation":[{"name":"National Institutes for Drug Control  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,24]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fitote.2019.104190"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1017\/S096042860000233X"},{"issue":"4","key":"e_1_2_11_4_1","first-page":"1","article-title":"To Explore the Safe Application of Tripterygium wilfordii Hook. f. Based on the Efficiency\/Toxicity Records in Ancient and Modern Literature","volume":"53","author":"Pei Q.","year":"2025","journal-title":"Liaoning Journal of Traditional Chinese Medicine"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3969\/j.issn.1672\u20103384.2019.05.005"},{"issue":"06","key":"e_1_2_11_6_1","first-page":"560","article-title":"Herbal Textual Research on Tripterygium wilfordii","volume":"7","author":"Gao W.","year":"2012","journal-title":"World Chinese Medicine"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27165102"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.13313\/j.issn.1673\u20104890.20201123001"},{"issue":"05","key":"e_1_2_11_9_1","first-page":"72","article-title":"HPLC\u2010ELSD Analysis of Five Terpenoid Compounds in Different Parts and Preparations of Tripterygium wilfordii","volume":"36","author":"Zhang L.","year":"2018","journal-title":"Journal of Sichuan Traditional Chinese Medicine"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.16155\/j.0254\u20101793.2013.03.013"},{"issue":"05","key":"e_1_2_11_11_1","first-page":"65","article-title":"Comparison on Content of Tripterine From Different Parts and Sources Upon Tripterygium wilfordii Hook. f","volume":"15","author":"Jiang J.","year":"2019","journal-title":"Asia Pacific Traditional Medicine"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.853023"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2016.02.001"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2022.10.009"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2023.1288943"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2025.116534"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1556\/168.2018.19.3.12"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13321\u2010015\u20100069\u20103"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2006.11.029"},{"key":"e_1_2_11_20_1","unstructured":"National Medical Products Administration.National Pharmaceutical Standards: WS3\u2010B\u20103350\u201398\u20132011. 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