{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T15:44:07Z","timestamp":1792079047654,"version":"4.4.0"},"reference-count":42,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:00:00Z","timestamp":1792022400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:00:00Z","timestamp":1792022400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100010049","name":"Kingston University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010049","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Analytical Science Advances"],"published-print":{"date-parts":[[2026,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Cassia alata<\/jats:italic>\n                    flowers remain phytochemically under\u2010characterized relative to the leaves. However, floral tissues can harbour distinct and, in some species, more abundant phenolic and flavonoid profiles, representing an unexploited source of antioxidant phytochemicals with potential functional\u2010food and nutraceutical relevance. Also,\n                    <jats:italic>Cassia alata<\/jats:italic>\n                    L. (Fabaceae) is a major source of numerous anthraquinones and flavonoids, yet the secondary metabolome of its floral tissues has not yet been thoroughly studied in comparison to its vegetative parts. This research therefore aimed to examine the phytoconstituents of\n                    <jats:italic>C. alata<\/jats:italic>\n                    flower extracts and evaluate their antioxidant potential. This represents, to our knowledge, the first liquid chromatography coupled with high\u2010resolution tandem mass spectrometry (LC\u2010HRMS\/MS) and 2,2\u2010diphenyl\u20101\u2010picrylhydrazyl (DPPH)\u2010based comparative profiling of\n                    <jats:italic>C. alata<\/jats:italic>\n                    flower extracts prepared with three solvents of differing polarity. Aqueous, 70% methanol, and 70% ethanol extracts of\n                    <jats:italic>C. alata<\/jats:italic>\n                    flowers were prepared and characterized using LC\u2010HRMS in negative ionization mode. The antioxidant potential was determined using the DPPH radical scavenging assay. LC\u2010HRMS enabled the tentative identification of 42 metabolites (in aqueous, methanolic, and ethanolic extracts), which were mainly flavonoids, phenolic acids, and glycosides. Some notable metabolites determined were Quercetin, Luteolin, Isomyricitin, Kaempferol\u2010dihexoside, Kaempferol\u2010\n                    <jats:italic>O<\/jats:italic>\n                    \u2010rutinoside, and Sophoricoside. The DPPH assay showed that all the extracts exhibited free radical scavenging activity in a concentration\u2010dependent manner. The ethanolic extract exhibited the strongest activity, with the lowest IC\n                    <jats:sub>50<\/jats:sub>\n                    value of 12.10 \u00b1 2.22\u00a0\u00b5g\/mL, followed by the aqueous extract, while the methanolic extract showed the weakest activity among the plant extracts. The observed antioxidant potential of the ethanolic extract may be associated with the wide diversity of tentatively identified flavonoid\u2010related metabolites. These results suggest that\u00a0\n                    <jats:italic>C. alata<\/jats:italic>\n                    \u00a0flower extracts may serve as potential sources of antioxidant phytochemicals for food and health\u2010related applications.\n                  <\/jats:p>","DOI":"10.1002\/ansa.70114","type":"journal-article","created":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T14:46:43Z","timestamp":1792075603000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Liquid Chromatography Coupled With High\u2010Resolution Mass Spectrometry Profiling of Metabolites in\n                    <i>Cassia alata<\/i>\n                    Flower Extracts and Their DPPH Free Radical Scavenging Activity"],"prefix":"10.1002","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-6317-104X","authenticated-orcid":false,"given":"Adonai Yin\u2010Anaab","family":"Kunduzore","sequence":"first","affiliation":[{"name":"School of Chemical and Biochemical Sciences University of Technology and Applied Sciences  Navrongo Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-1955-3273","authenticated-orcid":false,"given":"Shakira","family":"Ibrahim","sequence":"additional","affiliation":[{"name":"School of Chemical and Biochemical Sciences University of Technology and Applied Sciences  Navrongo Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Auphedeous Yinme","family":"Dang\u2010i","sequence":"additional","affiliation":[{"name":"School of Chemical and Biochemical Sciences University of Technology and Applied Sciences  Navrongo Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Adu\u2010Frimpong","sequence":"additional","affiliation":[{"name":"School of Chemical and Biochemical Sciences University of Technology and Applied Sciences  Navrongo Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7121-0783","authenticated-orcid":false,"given":"Salma","family":"Elshamy","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Biology German University in Cairo GUC  New Cairo City Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1681-8424","authenticated-orcid":false,"given":"Nikolai","family":"Kuhnert","sequence":"additional","affiliation":[{"name":"School of Science Constructor University Bremen  Bremen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9419-0014","authenticated-orcid":false,"given":"James","family":"Ziemah","sequence":"additional","affiliation":[{"name":"School of Chemical and Biochemical Sciences University of Technology and Applied Sciences  Navrongo Ghana"},{"name":"School of Science Constructor University Bremen  Bremen Germany"},{"name":"Department of Chemistry and Pharmaceutical Sciences HSSCE Faculty Kingston University  Kingston upon Thames UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,15]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1093\/jaoacint\/qsae028","article-title":"Integrated Multiplatform Analysis and Separation of Thirteen Flavonoids and Anthraquinones in Seven","volume":"107","author":"Saste G.","year":"2024","journal-title":"Journal of AOAC International"},{"issue":"1","key":"e_1_2_10_3_1","first-page":"4","article-title":"Asian Journal of Pharmaceutical Science & Technology HPLC Analysis and Antioxidant Potential of Plant Extract of Cassia Alata","volume":"4","author":"Sarkar B.","year":"2014","journal-title":"Asian Journal of Pharmaceutical Science and 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