{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T19:05:54Z","timestamp":1783537554832,"version":"3.55.0"},"reference-count":17,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,4,19]],"date-time":"2020-04-19T00:00:00Z","timestamp":1587254400000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["bsapubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Appl Plant Sci"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:sec><jats:title>Premise<\/jats:title><jats:p>Over 3000 species of plants and animals release toxic hydrogen cyanide (<jats:styled-content style=\"fixed-case\">HCN<\/jats:styled-content>) gas when their tissues are crushed. To investigate the role of cyanogenesis in <jats:italic>Passiflora<\/jats:italic>\u2013herbivore interactions, we developed an inexpensive, rapid, sensitive method for measuring <jats:styled-content style=\"fixed-case\">HCN<\/jats:styled-content> emissions from crushed tissues.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The method includes crushed tissue confinement in a closed chamber, where cyanogenesis reactions occur, followed by evacuation of gas to a portable <jats:styled-content style=\"fixed-case\">HCN<\/jats:styled-content> meter. Parts per million readings are repeated at 5\u2010min intervals until <jats:styled-content style=\"fixed-case\">HCN<\/jats:styled-content> is depleted. Three versions of the closed reaction chamber apparatus were tested: plastic cup, airtight combination mortar\u2010pestle, and glass desiccator jar.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We calibrated the method by comparing with a closed chamber measurement apparatus. The procedure's repeatability was demonstrated with a standard curve using known quantities of cyanogenic glycoside standard. Data collected with this method were also compared with the conventional colorimetric procedure. We processed over 2000 samples using this technique, revealing diverse elements of cyanogenic variation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These methods produced well\u2010defined data with minimal error. Results illustrated a one to four order\u2010of\u2010magnitude variation at organizational levels ranging from individual leaves to the entire <jats:italic>Passiflora<\/jats:italic> community. We now have a promising tool for uncovering the <jats:styled-content style=\"fixed-case\">HCN<\/jats:styled-content> phytochemical landscape in unprecedented detail.<\/jats:p><\/jats:sec>","DOI":"10.1002\/aps3.11336","type":"journal-article","created":{"date-parts":[[2020,4,19]],"date-time":"2020-04-19T04:50:11Z","timestamp":1587271811000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Using a portable hydrogen cyanide gas meter to uncover a dynamic phytochemical landscape"],"prefix":"10.1002","volume":"8","author":[{"given":"John","family":"Smiley","sequence":"first","affiliation":[{"name":"Office of Research Affairs University of California San Diego La Jolla California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2370-3617","authenticated-orcid":false,"given":"Colin R.","family":"Morrison","sequence":"additional","affiliation":[{"name":"Department of Integrative Biology Graduate Program in Ecology, Evolution and Behavior The University of Texas at Austin Austin Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,4,19]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2012.02.006"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12862-016-0843-5"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10886-006-9207-8"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/35018159"},{"key":"e_1_2_8_6_1","first-page":"403","volume-title":"Plant\u2010animal interactions: Evolutionary ecology in tropical and temperate regions","author":"Gilbert L. 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