{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T14:12:59Z","timestamp":1784902379934,"version":"3.55.0"},"reference-count":35,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,2,19]],"date-time":"2024-02-19T00:00:00Z","timestamp":1708300800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["bsapubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Appl Plant Sci"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Premise<\/jats:title><jats:p>With growing interest in the impact of false springs on plant reproduction, there is the need to develop reliable, high\u2010throughput methods for assessing floral freezing damage. Here we present a method for use with floral tissue that will facilitate more comparative work on floral freezing tolerance in the future.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and Results<\/jats:title><jats:p>We examined the effectiveness of a modified electrolyte leakage protocol to assess floral freezing damage. By comparing data from temperature response curves to an estimate of visual tissue damage, we optimized the protocol for different floral types and improved the signal\u2010to\u2010noise ratio for floral data.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our modified protocol provides a quick and straightforward method for quantifying floral freezing damage that can be standardized across floral types. This method allows for cross\u2010species comparisons and can be a powerful tool for studying broad patterns in floral freezing tolerance.<\/jats:p><\/jats:sec>","DOI":"10.1002\/aps3.11569","type":"journal-article","created":{"date-parts":[[2024,2,20]],"date-time":"2024-02-20T02:49:32Z","timestamp":1708397372000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Use of electrolyte leakage to assess floral damage after freezing"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7756-7166","authenticated-orcid":false,"given":"Jessica A.","family":"Savage","sequence":"first","affiliation":[{"name":"Department of Biology University of Minnesota Duluth Minnesota 55812 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sydney J.","family":"Hudzinski","sequence":"additional","affiliation":[{"name":"Department of Biology University of Minnesota Duluth Minnesota 55812 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mady R.","family":"Olson","sequence":"additional","affiliation":[{"name":"Department of Biology University of Minnesota Duluth Minnesota 55812 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,2,19]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1088\/1748-9326\/10\/10\/104008"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1890\/12-0200.1"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1086\/297568"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/treephys\/6.4.351"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/1365-2745.12482"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.21273\/HORTSCI.34.5.855"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.7.1.63"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.4141\/cjps67-043"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1752-4571.2008.00044.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.12813"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8137.2011.03992.x"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13007-021-00755-0"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.12452"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.21273\/JASHS.123.2.246"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.21273\/JASHS.125.4.429"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.2135\/cropsci1965.0011183X000500010024x"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1086\/314271"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00035-020-00236-5"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8137.1989.tb02408.x"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/treephys\/tpq059"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3054.2012.01616.x"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10530-020-02301-w"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-447602-8.50022-2"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.18893"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3040.2009.01991.x"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.2307\/1935383"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scienta.2014.04.002"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1890\/12-1779.1"},{"key":"e_1_2_8_30_1","unstructured":"Savage J. 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