{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:36:49Z","timestamp":1786977409385,"version":"build-2736575974"},"reference-count":43,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2018,11,12]],"date-time":"2018-11-12T00:00:00Z","timestamp":1541980800000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006338","name":"Vlaamse Interuniversitaire Raad","doi-asserted-by":"publisher","award":["2014RIP24"],"award-info":[{"award-number":["2014RIP24"]}],"id":[{"id":"10.13039\/501100006338","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bsapubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Appl Plant Sci"],"published-print":{"date-parts":[[2018,11]]},"abstract":"\n Premise of the Study<\/jats:title>\n \n The production of banana (\n Musa<\/jats:italic>\n spp.; Musaceae) plants is affected by various types of somaclonal variations (\n SV<\/jats:styled-content>\n ), including dwarfism. However, methods for specific detection of\n SV<\/jats:styled-content>\n are still scarce. To overcome this, a metabolite\u2010based method for detection of dwarf variants was evaluated.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n The gas chromatography\u2013mass spectrometry (\n GC<\/jats:styled-content>\n \u2010\n MS<\/jats:styled-content>\n ) metabolite profile of dwarf banana variants was investigated and compared to that of normal\u2010healthy (N) and cucumber mosaic virus (\n CMV<\/jats:styled-content>\n )\u2013infected plants using principal components analysis and partial least squares discriminant analysis (\n PLS<\/jats:styled-content>\n \u2010\n DA<\/jats:styled-content>\n ).\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Significant differences among the sample groups were observed in 82 metabolites. Rhamnose was exclusively present in dwarf plants but allothreonine and trehalose were present in all but\n SV<\/jats:styled-content>\n samples. Cellobiose was only detected in N plants, while 45 other metabolites, including methyl\u2010glucopyranoside, allopyranose, lactose, phenylalanine, and\n l<\/jats:sc>\n \u2010lysine were detected in all but\n CMV<\/jats:styled-content>\n \u2010infected samples.\n PLS<\/jats:styled-content>\n \u2010\n DA<\/jats:styled-content>\n models were able to detect\n SV<\/jats:styled-content>\n ,\n CMV<\/jats:styled-content>\n , and N plants with 100% accuracy and specificity.\n <\/jats:p>\n <\/jats:sec>\n \n Discussion<\/jats:title>\n \n The\n GC<\/jats:styled-content>\n \u2010\n MS<\/jats:styled-content>\n metabolite profile can be used for the rapid, specific detection of\n SV<\/jats:styled-content>\n at early plant production stages. This is the first metabolite\u2010based characterization and detection of somaclonal variation in plants.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/aps3.1194","type":"journal-article","created":{"date-parts":[[2018,11,12]],"date-time":"2018-11-12T10:15:43Z","timestamp":1542017743000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["GC<\/scp>\n \u2010\n MS<\/scp>\n metabolite profiling for specific detection of dwarf somaclonal variation in banana plants"],"prefix":"10.1002","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4609-7998","authenticated-orcid":false,"given":"Juan M.","family":"Cevallos\u2010Cevallos","sequence":"first","affiliation":[{"name":"Centro de Investigaciones Biotecnol\u00f3gicas del Ecuador Escuela Superior Polit\u00e9cnica del Litoral, ESPOL Campus Gustavo Galindo Km 30.5 V\u00eda Perimetral, P.O. Box 09\u201001\u20105863 Guayaquil Ecuador"},{"name":"Facultad de Ciencias de la Vida Escuela Superior Polit\u00e9cnica del Litoral, ESPOL Campus Gustavo Galindo Km 30.5 V\u00eda Perimetral, P.O. Box 09\u201001\u20105863 Guayaquil Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Jines","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones Biotecnol\u00f3gicas del Ecuador Escuela Superior Polit\u00e9cnica del Litoral, ESPOL Campus Gustavo Galindo Km 30.5 V\u00eda Perimetral, P.O. Box 09\u201001\u20105863 Guayaquil Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda G.","family":"Maridue\u00f1a\u2010Zavala","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones Biotecnol\u00f3gicas del Ecuador Escuela Superior Polit\u00e9cnica del Litoral, ESPOL Campus Gustavo Galindo Km 30.5 V\u00eda Perimetral, P.O. Box 09\u201001\u20105863 Guayaquil Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda J.","family":"Molina\u2010Miranda","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones Biotecnol\u00f3gicas del Ecuador Escuela Superior Polit\u00e9cnica del Litoral, ESPOL Campus Gustavo Galindo Km 30.5 V\u00eda Perimetral, P.O. Box 09\u201001\u20105863 Guayaquil Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel E.","family":"Ochoa","sequence":"additional","affiliation":[{"name":"Facultad de Ingenier\u00eda El\u00e9ctrica y Computaci\u00f3n Escuela Superior Polit\u00e9cnica del Litoral, ESPOL Campus Gustavo Galindo Km 30.5 V\u00eda Perimetral, P.O. 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