{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T02:38:10Z","timestamp":1787366290123,"version":"build-2736575974"},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,8,20]],"date-time":"2025-08-20T00:00:00Z","timestamp":1755648000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,8,20]],"date-time":"2025-08-20T00:00:00Z","timestamp":1755648000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["bsapubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Appl Plant Sci"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Premise<\/jats:title>\n                    <jats:p>Detecting clear tissue\u2010 and organ\u2010specific patterns of gene expression is key to understanding the genetic mechanisms that control plant development. In situ hybridization (ISH) of mRNA is one of the most precise, yet most challenging approaches to gene expression assays.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and Results<\/jats:title>\n                    <jats:p>\n                      Detection of histone H4 expression in reproductive tissues of\n                      <jats:italic>Mimulus lewisii<\/jats:italic>\n                      , a model angiosperm, was optimized using the RNAscope ISH assay. The optimized protocol was used to detect histone H4 expression in reproductive tissues of two gymnosperm species,\n                      <jats:italic>Taxodium distichum<\/jats:italic>\n                      and\n                      <jats:italic>Juniperus virginiana<\/jats:italic>\n                      , without further need for species\u2010specific optimization. Additionally, the optimized protocol was used to detect expression of\n                      <jats:italic>CYCLOIDEA<\/jats:italic>\n                      transcription factors in\n                      <jats:italic>M. lewisii<\/jats:italic>\n                      reproductive tissues without further optimization and with results similar to those previously reported.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The RNAscope assay can quickly and sensitively generate high\u2010quality ISH results in reproductive tissues across a breadth of plant species.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/aps3.70020","type":"journal-article","created":{"date-parts":[[2025,8,20]],"date-time":"2025-08-20T08:54:41Z","timestamp":1755680081000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Expanded application to plant reproductive tissues of a branched DNA probe\u2010based in situ hybridization method"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-5434-3403","authenticated-orcid":false,"given":"Brooklyn M.","family":"Anaya","sequence":"first","affiliation":[{"name":"Department of Ecology and Evolutionary Biology University of Kansas Lawrence 66045 Kansas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-5644-726X","authenticated-orcid":false,"given":"Austin T.","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Ecology and Evolutionary Biology University of Kansas Lawrence 66045 Kansas USA"},{"name":"Biodiversity Institute University of Kansas Lawrence 66045 Kansas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9533-2934","authenticated-orcid":false,"given":"Kelly K. S.","family":"Matsunaga","sequence":"additional","affiliation":[{"name":"Department of Ecology and Evolutionary Biology University of Kansas Lawrence 66045 Kansas USA"},{"name":"Biodiversity Institute University of Kansas Lawrence 66045 Kansas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6116-666X","authenticated-orcid":false,"given":"Lena C.","family":"Hileman","sequence":"additional","affiliation":[{"name":"Department of Ecology and Evolutionary Biology University of Kansas Lawrence 66045 Kansas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,20]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.25606"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2016.01.017"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.001941"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3732\/apps.1400011"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00198694"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.12360"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0300-9084(93)90057-Y"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajb2.16271"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1525-142X.2011.00467.x"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.ado4571"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants10071423"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1525-142X.2011.00466.x"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1835725100"},{"key":"e_1_2_8_16_1","doi-asserted-by":"crossref","unstructured":"Hileman L. 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