{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T14:58:01Z","timestamp":1787324281265,"version":"build-2736575974"},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T00:00:00Z","timestamp":1778112000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T00:00:00Z","timestamp":1778112000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["bsapubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Appl Plant Sci"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Premise<\/jats:title>\n                    <jats:p>Third\u2010generation sequencing has revolutionized genomics, enabling in\u2010depth analysis of genome sequence, structure, and epigenetic features. Yet, extracting high\u2010quality DNA for long\u2010read sequencing remains a bottleneck\u2014particularly in non\u2010model plants, such as mature trees growing in natural environments, which often contain abundant endogenous compounds that hinder extraction and downstream applications.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and Results<\/jats:title>\n                    <jats:p>\n                      We developed an optimized, robust, and cost\u2010effective DNA extraction protocol that yields high\u2010quality DNA suitable for Oxford Nanopore Technologies and PacBio sequencing. Validation across diverse taxa\u2014including six\n                      <jats:italic>Nothofagus<\/jats:italic>\n                      species, gymnosperms endemic to Andean\u2013Patagonian forests, exotic conifers of commercial value, and model plants\u2014demonstrated consistently high DNA purity (A\n                      <jats:sub>260<\/jats:sub>\n                      \/A\n                      <jats:sub>280<\/jats:sub>\n                      \u2009&gt;\u20091.8, A\n                      <jats:sub>260<\/jats:sub>\n                      \/A\n                      <jats:sub>230<\/jats:sub>\n                      \u2009&gt;\u20092.0) and fragment sizes \u226530 kbp. Downstream sequencing confirmed suitability for applications requiring long, intact molecules and base modification detection.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Compared to commercial kits and standard protocols, this approach achieved superior DNA integrity and yield without specialized equipment, offering an accessible solution for researchers working with challenging plant species.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/aps3.70055","type":"journal-article","created":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T23:35:27Z","timestamp":1778196927000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A cost\u2010effective DNA extraction protocol for long\u2010read sequencing in non\u2010model plants"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-7420-0616","authenticated-orcid":false,"given":"Sofia","family":"Gaischuk","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones Forestales y Agropecuarias Bariloche, Instituto Nacional de Tecnolog\u00eda Agropecuaria, Estaci\u00f3n Experimental Bariloche \u2013 Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (INTA EEA Bariloche\u2010CONICET) San Carlos de Bariloche R8403DVZ R\u00edo Negro Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9007-2398","authenticated-orcid":false,"given":"Cintia F.","family":"Marchetti","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Forestales y Agropecuarias Bariloche, Instituto Nacional de Tecnolog\u00eda Agropecuaria, Estaci\u00f3n Experimental Bariloche \u2013 Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (INTA EEA Bariloche\u2010CONICET) San Carlos de Bariloche R8403DVZ R\u00edo Negro Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0025-3708","authenticated-orcid":false,"given":"Ezequiel","family":"Petrillo","sequence":"additional","affiliation":[{"name":"Instituto de Fisiolog\u00eda, Biolog\u00eda Molecular y Neurociencias, CONICET\u2013Universidad de Buenos Aires Buenos Aires C1428EHA Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1538-9752","authenticated-orcid":false,"given":"Fernando A.","family":"Rabanal","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology Max Planck Institute for Biology T\u00fcbingen T\u00fcbingen 72076 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2114-7963","authenticated-orcid":false,"given":"Detlef","family":"Weigel","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology Max Planck Institute for Biology T\u00fcbingen T\u00fcbingen 72076 Germany"},{"name":"Institute for Bioinformatics and Medical Informatics University of T\u00fcbingen T\u00fcbingen 72076 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6637-3465","authenticated-orcid":false,"given":"Nicol\u00e1s","family":"Bellora","sequence":"additional","affiliation":[{"name":"Laboratorio de Gen\u00f3mica Computacional, Instituto de Tecnolog\u00edas Nucleares para la Salud, Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas San Carlos de Bariloche 19 8400 Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9483-7906","authenticated-orcid":false,"given":"M. Ver\u00f3nica","family":"Arana","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Forestales y Agropecuarias Bariloche, Instituto Nacional de Tecnolog\u00eda Agropecuaria, Estaci\u00f3n Experimental Bariloche \u2013 Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (INTA EEA Bariloche\u2010CONICET) San Carlos de Bariloche R8403DVZ R\u00edo Negro Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,7]]},"reference":[{"issue":"1","key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1186\/s42269-019-0066-1","article-title":"Extraction of high\u2010quality genomic DNA from different plant orders applying a modified CTAB\u2010based method","volume":"43","author":"Aboul\u2010Maaty N. 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