{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T14:58:38Z","timestamp":1787324318531,"version":"build-2736575974"},"reference-count":48,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T00:00:00Z","timestamp":1784764800000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["bsapubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Appl Plant Sci"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Premise<\/jats:title>\n                    <jats:p>Genomic analysis of population structure is important for understanding plant genetic diversity among and within populations. A limitation to the broader application of genetics in conservation and related fields is the cost of obtaining large datasets. Targeted sequencing and low\u2010volume robotic liquid handlers can reduce library preparation reaction volumes and costs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We used targeted sequencing via Angiosperms353 to obtain data for 768 samples, 18 of which were identical, at 0.5\u00d7 and 0.1\u00d7 reaction volumes. We calculated quality and quantity control statistics to compare the effects of tissue age and library reaction volume on sequencing results for on\u2010target nuclear and off\u2010target plastid genes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Library miniaturization to 0.1\u00d7 reduces costs and generally performs comparably to 0.5\u00d7 libraries. In the full dataset, Tenth\u2010Volume Only libraries showed smaller insert sizes and fewer genes with mapped sequences, but no reduction in mapped reads. In the Overlap Set, 0.1\u00d7 libraries had equal or improved performance with no significant decrease in sequencing efficiency. Differences by tissue type likely reflected sampling variation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>Miniaturization to 0.1\u00d7 substantially reduces per\u2010sample costs while maintaining comparable sequencing quality across fresh and herbarium angiosperm DNA. Overall, miniaturization provides a reliable, cost\u2010effective approach for targeted sequencing, increasing the feasibility of using herbarium collections and enabling broader access to population\u2010level genomic studies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/aps3.70070","type":"journal-article","created":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T09:32:47Z","timestamp":1784799167000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Fun\u2010sized library prep: Miniaturization is a valid method for per\u2010sample cost reduction in targeted sequencing of angiosperm DNA"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9511-0170","authenticated-orcid":false,"given":"Madison R.","family":"Bullock","sequence":"first","affiliation":[{"name":"Department of Biological Sciences Texas Tech University 2901 Main Street Lubbock 79409 Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"Fokar","sequence":"additional","affiliation":[{"name":"Center for Biotechnology and Genomics Texas Tech University 1008 Canton Avenue Lubbock 79409 Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rylee D.","family":"Creek","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Texas Tech University 2901 Main Street Lubbock 79409 Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliot A.","family":"Stevens","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Texas Tech University 2901 Main Street Lubbock 79409 Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1958-6334","authenticated-orcid":false,"given":"Matthew G.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Texas Tech University 2901 Main Street Lubbock 79409 Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,23]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1600\/036364421X16370109698740"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.csbj.2019.10.004"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/acprof:oso\/9780199554232.003.0017"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/aps3.11406"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2019.01102"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty560"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/conl.12619"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abb4484"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10592-005-9073-x"},{"key":"e_1_2_8_11_1","unstructured":"Eccles R. X.Liu C.Nelson S.Haldenby F.Johnson J.Fothergill K.Koprowska et al.2023. Miniaturisation of DNA library preparation for Illumina sequencing using mosquito\u00ae liquid handler.https:\/\/www.sptlabtech.com\/hubfs\/6586853\/SPT%20Labtech%20Website\/09%20-%20Resources\/SPT%20Labtech%20-%20Miniaturisation%20Liverpool%20University%20App%20Note.pdf[accessed 30 June 2026]."},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1739.1995.09030686.x"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocon.2010.05.011"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.3732\/apps.1600017"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2026347118"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/aps3.11337"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-49679-y"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/life12111871"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1461-0248.2008.01179.x"},{"key":"e_1_2_8_20_1","doi-asserted-by":"crossref","unstructured":"Johnson B. K. M. F.Majewski H. J.Jang R. A.Siwicki M.Wegener A.Semwal J.Morrison et al.2025. Efficient profiling of total RNA in single cells with STORM\u2010seq. bioRxiv 2022.03.14.484332 [preprint]. Available athttps:\/\/doi.org\/10.1101\/2022.03.14.484332[posted 20 May 2025; accessed 29 June 2026].","DOI":"10.1101\/2022.03.14.484332"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.3732\/apps.1600016"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/sysbio\/syy086"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-05049-6"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2019.01655"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-09454-w"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/ecog.01507"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.3732\/apps.1300085"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/aps3.1038"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/aps3.11568"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1177\/2211068216630741"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.15732"},{"key":"e_1_2_8_32_1","doi-asserted-by":"crossref","unstructured":"Ogiso\u2010Tanaka E. A.Kaga andM.Hajika.2018. Miniaturization technologies for cost\u2010effective AmpliSeq library preparation for next\u2010generation sequencing. bioRxiv 367464 [preprint]. Available athttps:\/\/doi.org\/10.1101\/467464[posted 8 December 2018; accessed 18 June 2026].","DOI":"10.1101\/467464"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth1107-891"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0283219"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2024.1340056"},{"key":"e_1_2_8_36_1","unstructured":"Python Software Foundation.2025. Python (Version 3.12) [Computer software].https:\/\/www.python.org\/."},{"key":"e_1_2_8_37_1","volume-title":"R: A language and environment for statistical computing","author":"R Core Team","year":"2026"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.128124.111"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes13040707"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/conl.12564"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2020.00258"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/aps3.11419"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1744-7348.1996.tb07096.x"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/csp2.218"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gecco.2017.04.001"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.15312"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-35087-7"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.3732\/apps.1400042"},{"key":"e_1_2_8_49_1","doi-asserted-by":"crossref","unstructured":"Y\u00e1ng\u00fcez E. G.White S.Kreutzer L.Opitz L.Poveda T.Sykes M. D.Moccia et al.2020. HiDRA\u2010seq: High\u2010throughput SARS\u2010CoV\u20102 detection by RNA barcoding and amplicon sequencing. bioRxiv 130484 [preprint]. Available athttps:\/\/doi.org\/10.1101\/2020.06.02.130484[posted 2 June 2020; accessed 18 June 2026].","DOI":"10.1101\/2020.06.02.130484"}],"container-title":["Applications in Plant Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/bsapubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/aps3.70070","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bsapubs.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/aps3.70070","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bsapubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/aps3.70070","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T14:21:54Z","timestamp":1787322114000},"score":1,"resource":{"primary":{"URL":"https:\/\/bsapubs.onlinelibrary.wiley.com\/doi\/10.1002\/aps3.70070"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7]]},"references-count":48,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1002\/aps3.70070"],"URL":"https:\/\/doi.org\/10.1002\/aps3.70070","archive":["Portico"],"relation":{},"ISSN":["2168-0450","2168-0450"],"issn-type":[{"value":"2168-0450","type":"print"},{"value":"2168-0450","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7]]},"assertion":[{"value":"2025-09-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70070"}}