{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T10:43:25Z","timestamp":1789469005427,"version":"build-2803163510"},"reference-count":42,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["bsapubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Appl Plant Sci"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Premise<\/jats:title>\n                    <jats:p>Herbarium specimens are increasingly used to extract morphological traits for ecological and evolutionary studies, yet the effects of tissue desiccation on trait measurements remain poorly understood. Here, we tested two hypotheses: (H1) whether higher tissue water content leads to greater measurement changes after herborization and (H2) whether fresh trait values can be reliably predicted from measurements of herborized specimens.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We evaluated the reliability of herbarium\u2010based measurements by comparing fresh and dried traits of leaves, flowers, fleshy fruits, and seeds across 262 individuals representing 133 Neotropical Myrtaceae species. Phylogenetic least squares models and machine learning regressions were used to test H1 and H2.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Leaves and flowers generally shrank after herborization, fruits size metrics tended to increase, and seeds were largely unaffected. Water content was significantly associated with the magnitude of herborization effects in flowers and some leaf and seed traits. Fresh trait values were accurately predicted from measurements of herborized specimens. Prediction errors were lowest for leaf traits, followed by fruits, flowers, and seeds.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>These results partially support H1 and support H2, indicating that herborized specimens can be reliably used for trait analyses when organ\u2010specific responses are considered, providing a practical framework to account for potential desiccation bias in functional trait research.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/aps3.70082","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T09:22:14Z","timestamp":1789464134000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Herbarium\u2010based measurements are reliable predictors of fresh plant traits in Neotropical Myrtaceae"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-6909-0769","authenticated-orcid":false,"given":"Yacov","family":"Kilsztajn","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Ecologia Universidade Federal do Rio Grande do Norte Natal 59078\u2212900 Rio Grande do Norte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9804-3476","authenticated-orcid":false,"given":"L\u00e1zaro Henrique Soares Moraes de","family":"Concei\u00e7\u00e3o","sequence":"additional","affiliation":[{"name":"Departamento de Bot\u00e2nica Universidade de Bras\u00edlia, Campus Darcy Ribeiro Bras\u00edlia 70910\u2212900 DF Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9991-7924","authenticated-orcid":false,"given":"Thais","family":"Vasconcelos","sequence":"additional","affiliation":[{"name":"Department of Ecology and Evolutionary Biology University of Michigan Ann Arbor 48109 Michigan USA"},{"name":"University of Michigan Herbarium, University of Michigan Ann Arbor 48108 Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8924-2692","authenticated-orcid":false,"given":"Carolyn Elinore Barnes","family":"Proen\u00e7a","sequence":"additional","affiliation":[{"name":"Departamento de Bot\u00e2nica Universidade de Bras\u00edlia, Campus Darcy Ribeiro Bras\u00edlia 70910\u2212900 DF Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4911-9574","authenticated-orcid":false,"given":"Vanessa Graziele","family":"Staggemeier","sequence":"additional","affiliation":[{"name":"LEVES \u2010 Laborat\u00f3rio de Ecologia Vegetal, Evolu\u00e7\u00e3o e S\u00edntese, Departamento de Ecologia Universidade Federal do Rio Grande do Norte Natal 59078\u2212900 Rio Grande do Norte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,15]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"27","volume-title":"Seeds: Physiology of development, germination and dormancy","author":"Bewley J. 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