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At 2 weeks of age, weak Hsp27 immunoreactivity was distributed diffusely over all gland components. At 3 weeks, Hsp27 immunoreactivity disappeared in most parts of the acini and ducts, but was intensely accumulated in a small cell population located in the acinar center. This population was composed mostly of terminal tubule (TT) type I cells. At 4 weeks, the Hsp27\u2010immunopositive cell population in the acinar center was composed primarily of immature (type II) acinar cells, partly of immature (granulated) intercalated duct (ID) cells, and occasionally of apoptotic cells. After 5 weeks, all acinar components became mature and were no longer immunoreactive for Hsp27. When acinar cell differentiation was accelerated by administration of isoproterenol to 3\u2010week\u2010old rats for 7 days, the number of Hsp27\u2010positive cells was significantly lower than in the control gland at 4 weeks, confirming that Hsp27 expression is downregulated in mature acinar cells. These results suggest that at around 3\u20134 weeks in postnatal development, the centroacinar TT cells stop proliferating and begin to differentiate into acinar and ID cells, and occasionally undergo apoptosis. Hsp27 is transiently expressed in the centroacinar TT cells during this critical period, and thus may play a role in their differentiation into the immediate descendants. Anat Rec 264:358\u2013366, 2001. \u00a9 2001 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ar.10023","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T21:17:12Z","timestamp":1030310232000},"page":"358-366","source":"Crossref","is-referenced-by-count":12,"title":["Transient occurrence of 27 kDa heat\u2010shock protein in the terminal tubule cells during postnatal development of the rat submandibular gland"],"prefix":"10.1002","volume":"264","author":[{"given":"Osamu","family":"Amano","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshinori","family":"Kudo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mayumi","family":"Shimada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomohiko","family":"Wakayama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miyuki","family":"Yamamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shoichi","family":"Iseki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,10,30]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1679\/aohc1950.38.177"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1003258514766"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1267\/ahc.34.111"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.3109\/08977199409010988"},{"key":"e_1_2_6_6_1","volume-title":"The biology of heart shock proteins and molecular chaperons","author":"Arrigo A\u2010P","year":"1994"},{"key":"e_1_2_6_7_1","series-title":"Neuroscience Perspectives Series","first-page":"145","volume-title":"Heat shock or cell stress proteins in the nervous system","author":"Arrigo A\u2010P","year":"1994"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-58259-2_5"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/28.8.7003006"},{"key":"e_1_2_6_10_1","first-page":"225","article-title":"Purification of the growth\u2010related protein p25 of the Ehrlich ascites tumor and analysis of its isoforms","volume":"17","author":"Benndorf R","year":"1988","journal-title":"Biochem Int"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1091780204"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1091780205"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/85.19.1558"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(74)90280-2"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.1051400307"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1999.9596"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(72)90041-3"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.1020140204"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/355033a0"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1177\/28.8.6160181"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00225529"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.8.3314"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1177\/002215540004800513"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0185(20000401)258:4<349::AID-AR3>3.0.CO;2-9"},{"key":"e_1_2_6_25_1","first-page":"201","article-title":"The post\u2010natal development of the rat submaxillary gland","volume":"93","author":"Jacoby F","year":"1959","journal-title":"J Anat"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)53882-5"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(84)90626-5"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1177\/002215549804601109"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(20)80512-2"},{"key":"e_1_2_6_30_1","first-page":"287","article-title":"An electron microscopic study of the rat submaxillary gland during its post\u2010natal development and in the adult","volume":"93","author":"Leeson CR","year":"1959","journal-title":"J Anat"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ge.22.120188.003215"},{"key":"e_1_2_6_32_1","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1007\/BF02899735","article-title":"The postnatal development of myoepithelial cells in the rat submandibular gland. 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