{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:31:04Z","timestamp":1786977064625,"version":"build-2736575974"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2001,12,5]],"date-time":"2001-12-05T00:00:00Z","timestamp":1007510400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Anat. Rec."],"published-print":{"date-parts":[[2002,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Cell differentiation in the rat sublingual gland occurs rapidly and is largely complete by birth. To study differentiation of the serous and mucous cells of the sublingual gland, we used antibodies to the secretory proteins CSP\u20101, SMGB, PSP, and SMGD, and sublingual mucin as specific cell markers. Glands from rats at ages 18, 19, and 20 days in utero, and postnatal days 0, 1, 5, 9, 14, 18, 25, 40, and 60 were fixed and prepared for morphological analysis and immunocytochemical labeling. At age 18 days in utero, a few cells in the developing terminal bulbs contained mucous\u2010like apical granules that labeled with anti\u2010mucin. Other cells had mixed granules with a peripheral lucent region and a dense core of variable size that occasionally labeled with anti\u2010SMGD. Additionally, presumptive serous cells with small dense granules that contained CSP\u20101 and SMGB were present. At age 19 days in utero, the dense granules of these cells also labeled with anti\u2010SMGD. By age 20 days in utero, mucous cells were filled with large, pale granules that labeled with anti\u2010mucin, and serous cells had numerous dense granules containing CSP\u20101, SMGB, PSP, and SMGD. Fewer cells with mixed granules were seen, but dense regions present in some mucous granules (MGs) labeled with anti\u2010SMGD. After birth, fewer MGs had dense regions, and serous cells were organized into well\u2010formed demilunes. Except for PSP, which was undetectable after the fifth postnatal day, the pattern of immunoreactivity observed in glands of neonatal and adult animals was similar to that seen by age 20 days in utero. These results suggest that mucous and serous cells have separate developmental origins, mucous cells differentiate earlier than serous cells, and cells with mixed granules may become mucous cells. Anat Rec 266:30\u201342, 2002. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ar.10027","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T12:27:19Z","timestamp":1030278439000},"page":"30-42","source":"Crossref","is-referenced-by-count":30,"title":["Development of the rat sublingual gland: A light and electron microscopic immunocytochemical study"],"prefix":"10.1002","volume":"266","author":[{"given":"M.S.","family":"Wolff","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Mirels","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Lagner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.R.","family":"Hand","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2001,12,7]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1679\/aohc1950.38.177"},{"key":"e_1_2_6_3_1","first-page":"112","article-title":"Two independently regulated secretory systems within the acini of the submandibular gland of the perinatal rat","volume":"33","author":"Ball WD","year":"1984","journal-title":"Eur J Cell Biol"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(88)90210-2"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/39.12.1940321"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/10454411930040033701"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1091780204"},{"key":"e_1_2_6_8_1","first-page":"617","article-title":"Acidic epididymal glycoprotein is a major secretory protein of rat parotid gland","volume":"4","author":"Charest NJ","year":"1993","journal-title":"Crit Rev Oral Biol Med"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(74)90280-2"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/10454411970080010301"},{"key":"e_1_2_6_11_1","doi-asserted-by":"crossref","first-page":"26592","DOI":"10.1016\/S0021-9258(19)74353-1","article-title":"Characterization of common salivary protein 1, a product of rat submandibular, sublingual and parotid glands","volume":"268","author":"Girard L","year":"1993","journal-title":"J Biol Chem"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-012286230-4\/50006-7"},{"key":"e_1_2_6_13_1","first-page":"187","article-title":"A new mutation involving the sublingual gland in NFS\/N mice. 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