{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,1,21]],"date-time":"2024-01-21T15:53:00Z","timestamp":1705852380681},"reference-count":48,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,1,27]],"date-time":"2005-01-27T00:00:00Z","timestamp":1106784000000},"content-version":"vor","delay-in-days":12445,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Anat. Rec."],"published-print":{"date-parts":[[1971,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The intracellular distribution of mucosubstances in the acinar cells of the rat sublingual gland has been studied at the electron microscopic level by means of two histochemical techniques: PA\u2010silver methenamine for the detection of vicinal glycols and colloidal thorium for the detection of polyanions. By both methods the secretory granules in the mucous acinar cells were selectively stained. The reaction products were visualized as dense closely packed precipitates covering the entire matrix of the individual mucous granules. Similar reaction products were also often observed within the Golgi apparatus of the mucous cells. In striking contrast, the secretory granules and Golgi apparatus of the demilunar cells exhibited no demonstrable selective staining by either method. It is concluded that the secretory granules of the mucous acinar cells are largely composed of mucosubstances containing vicinal glycols and polyanions and that the secretory granules in demilunar cells contain no demonstrable content of such mucosubstances.<\/jats:p>","DOI":"10.1002\/ar.1091690108","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T09:06:52Z","timestamp":1109408812000},"page":"71-95","source":"Crossref","is-referenced-by-count":14,"title":["Intracellular distribution of mucosubstances in the major sublingual gland of the rat"],"prefix":"10.1002","volume":"169","author":[{"given":"Shoji","family":"Enomoto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bronnetta L.","family":"Scott","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,1,27]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.5.3.508"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.6.1.113"},{"key":"e_1_2_1_4_1","first-page":"1","article-title":"Studies on sialic acids","volume":"61","author":"Blix G.","year":"1956","journal-title":"Acta Soc. Med. Upsal."},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.2.4.15"},{"key":"e_1_2_1_6_1","first-page":"457","article-title":"Studies on paraformaldehyde fixation for electron microscopy","volume":"6","author":"Burgos M. H.","year":"1967","journal-title":"J. Microscopie"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.3.5.827"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.4.6.841"},{"key":"e_1_2_1_9_1","first-page":"427","article-title":"Acid mucopolysaccharides in electron microscopy; the use of the colloidal iron method","volume":"99","author":"Curran R. C.","year":"1965","journal-title":"J. Anat."},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5320(67)80221-1"},{"key":"e_1_2_1_11_1","first-page":"423","article-title":"Die qualitative elektronenmikroskopische Darstellung von Stoffen mit der Gruppe CHOH\u2010CHOH. Ein Beitrag zur Elektronenf\u00e4rbung","volume":"61","author":"Dettmer N.","year":"1953","journal-title":"Z. wiss. Mikr."},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.19.1.223"},{"key":"e_1_2_1_13_1","doi-asserted-by":"crossref","first-page":"50","DOI":"10.5962\/bhl.title.6273","volume-title":"Microscopic Histochemistry; Principles and Practice","author":"Gomori G.","year":"1952"},{"key":"e_1_2_1_14_1","first-page":"12","volume-title":"The chemistry and biology of sialic acids and related substances","author":"Gottschalk A.","year":"1960"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1177\/5.5.445"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1177\/15.12.745"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.9.2.409"},{"key":"e_1_2_1_18_1","first-page":"54A","article-title":"Electron microscopy of cell extraneous coats as revealed by ruthenium red staining","volume":"23","author":"Luft J. H.","year":"1964","journal-title":"J. Cell Biol."},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5320(68)90014-2"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.9.1.121"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1177\/6.5.309"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-3233(08)60626-9"},{"key":"e_1_2_1_23_1","first-page":"1637","article-title":"Advantages of a phosphate buffer for Os O4 solutions in fixation","volume":"32","author":"Millonig G.","year":"1961","journal-title":"J. Appl. Phys."},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/35.6.528"},{"key":"e_1_2_1_25_1","first-page":"566","article-title":"Improved procedure for the staining of acidic polysaccharides by M\u00fclle r' s colloidal\n(hydrous) ferric oxide and its combination with the periodic acid\u2010Schiff reactions","volume":"7","author":"Mowry R. W.","year":"1958","journal-title":"Lab. Invest."},{"key":"e_1_2_1_26_1","first-page":"288","article-title":"Effect of periodic acid used prior to chromic acid on the staining of polysaccharides by Gomori's methenamine silver","volume":"7","author":"Mowry R. W.","year":"1959","journal-title":"J. Histochem. Cytochem."},{"key":"e_1_2_1_27_1","first-page":"342","article-title":"Buffered formaldehyde as a killing agent and primary fixative for electron microscopy","volume":"142","author":"Pease D. C.","year":"1962","journal-title":"Anat. Rec."},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5320(66)80054-0"},{"key":"e_1_2_1_29_1","first-page":"502","article-title":"The nature of epithelial mucins","volume":"9","author":"Pigman W.","year":"1959","journal-title":"Int. Dent. J."},{"key":"e_1_2_1_30_1","first-page":"1","article-title":"Histochemical studies on human mucous secreting salivary glands","volume":"12","author":"Quintarelli G.","year":"1961","journal-title":"Acta Histochemie"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.40.2.395"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.32.1.27"},{"key":"e_1_2_1_33_1","first-page":"535","article-title":"A stain for the ultrastructural localization of acid mucopolysaccharides","volume":"3","author":"Revel J. P.","year":"1964","journal-title":"J. Microscopie"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(66)90275-8"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.17.1.19"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001040106"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-1-4832-2871-6.50009-6"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5320(69)90009-4"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001110104"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1091490304"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1177\/13.7.599"},{"key":"e_1_2_1_42_1","first-page":"0","volume-title":"5th International Congress for Electron Microscopy","author":"Swift H.","year":"1962"},{"key":"e_1_2_1_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5320(67)80017-0"},{"key":"e_1_2_1_44_1","doi-asserted-by":"publisher","DOI":"10.1177\/13.4.286"},{"key":"e_1_2_1_45_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.25.2.407"},{"key":"e_1_2_1_46_1","first-page":"251","article-title":"Histochemical detection of mucopolysaccharides in the major salivary glands of the rat","volume":"9","author":"Villa N.","year":"1964","journal-title":"Ann. Histochim."},{"key":"e_1_2_1_47_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.30.2.299"},{"key":"e_1_2_1_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(57)90137-4"},{"key":"e_1_2_1_49_1","first-page":"501","article-title":"Fibroblasts in tissue culture: Use of colloidal iron for ultrastructural localization of acid mucopolysaccharides","volume":"14","author":"Yardley J. H.","year":"1965","journal-title":"Lab. Invest."}],"container-title":["The Anatomical Record"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Far.1091690108","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ar.1091690108","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,12]],"date-time":"2023-11-12T07:24:23Z","timestamp":1699773863000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ar.1091690108"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1971,1]]},"references-count":48,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1971,1]]}},"alternative-id":["10.1002\/ar.1091690108"],"URL":"https:\/\/doi.org\/10.1002\/ar.1091690108","archive":["Portico"],"relation":{},"ISSN":["0003-276X","1097-0185"],"issn-type":[{"value":"0003-276X","type":"print"},{"value":"1097-0185","type":"electronic"}],"subject":[],"published":{"date-parts":[[1971,1]]}}}