{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,12]],"date-time":"2023-11-12T14:13:44Z","timestamp":1699798424959},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,1,27]],"date-time":"2005-01-27T00:00:00Z","timestamp":1106784000000},"content-version":"vor","delay-in-days":12325,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Anat. Rec."],"published-print":{"date-parts":[[1971,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Buffy coats from blood and bone marrow were fixed in phosphate\u2010buffered glutaraldehyde, exposed to the osmium\u2010zinc iodide (OZI) reagent for 24 hours, dehydrated and embedded in Epon 812. OZI reactivity of blood and bone marrow cells was selectively confined to the cisternae of the rough endoplasmic reticulum (RER), Golgi complex, nuclear envelope and to the mito\u2010chondrial matrices; membranes and other organelles were non\u2010reactive. Some variation in intensity and distribution of OZI reactivity was evident within individual cells and organelles. Continuities between the cisternae of the nuclear envelope and RER as well as between these cisternae and those of the Golgi complex were more conspicuous in OZI preparations than in specimens prepared for routine electron microscopy.<\/jats:p><jats:p>The amount and distribution of the cisternal elements and mitochondria within the developing leukocytes and erythrocytes of the bone marrow were evaluated using the OZI technique. All leukocyte granules and their precursor forms fail to stain with the OZI reagent; portions of the Golgi complex most closely associated with the packaging of the cytoplasmic granules also are non\u2010reactive following exposure to the OZI reagent. Reactivity is absent in mature erythrocytes while mitochondria and cisternal components of immature erythroid cells yield positive OZI reactions. Heat, methanol and cyanide inhibit OZI reactivity while a dimorphism of OZI staining is induced between mitochondria and cisternal components by N\u2010ethylmaleimide.<\/jats:p>","DOI":"10.1002\/ar.1091700107","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T09:12:35Z","timestamp":1109409155000},"page":"81-95","source":"Crossref","is-referenced-by-count":19,"title":["Osmium\u2010zinc iodide reactivity in human blood and bone marrow cells"],"prefix":"10.1002","volume":"170","author":[{"given":"Michael A.","family":"Clark","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. Adolph","family":"Ackerman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,1,27]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1964.tb40690.x"},{"key":"e_1_2_1_3_1","first-page":"290","article-title":"Ultrastructure and cytochemistry of the developing neutrophil","volume":"19","author":"Ackerman G. A.","year":"1968","journal-title":"Lab. Invest."},{"key":"e_1_2_1_4_1","first-page":"269","article-title":"Ultra\u2010structural cytochemistry of human blood and bone marrow cells examined by the osmiumzinc iodide method","volume":"166","author":"Ackerman G. A.","year":"1970","journal-title":"Anat. 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