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The dose which optimises survival and paralysis is determined along with the effect of the drugs on embryonic growth. Decamethonium iodide at a dose of 1 mg per embryo gave maximum survival and paralysis to 18 days of incubation. Paralysis was assessed by observation of treated embryos <jats:italic>in ovo<\/jats:italic> and by examination of embryos removed from their shells between 11 and 18 days of incubation. Embryos were completely paralysed 24 hours post\u2010injection and remained paralysed until 18 days of incubation. Paralysed embryos failed to hatch. Development of the leg musculature was severely retarded in paralysed embryos. This method of inducing paralysis has considerable advantages over previous continuous infusion methods. The growth and collagen content of the tibia in the paralysed embryos was reduced and these results, and other applications of the method, are discussed.<\/jats:p>","DOI":"10.1002\/ar.1091810408","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T08:00:36Z","timestamp":1109404836000},"page":"767-777","source":"Crossref","is-referenced-by-count":27,"title":["A simple, single\u2010injection method for inducing long\u2010term paralysis in embryonic chicks, and preliminary observations on growth of the tibia"],"prefix":"10.1002","volume":"181","author":[{"given":"Brian K.","family":"Hall","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,1,27]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1091550115"},{"key":"e_1_2_1_3_1","first-page":"209","volume-title":"Bone Biodynamics","author":"Becker R. 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