{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T18:42:10Z","timestamp":1774464130483,"version":"3.50.1"},"reference-count":23,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,1,26]],"date-time":"2005-01-26T00:00:00Z","timestamp":1106697600000},"content-version":"vor","delay-in-days":9614,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Anat. Rec."],"published-print":{"date-parts":[[1978,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Epithelial cells of the several subtypes that comprise the small\u2010granule cell population of the respiratory system are little studied, partly because adequate silver, monoamine fluorescence and other specific light microscopical preparations have been more difficult to obtain than in the gut and other organs possessing diffuse endocrine systems. Periodic acid\u2010Schiff (PAS) in combination with MacConaill\u2010Solcia's lead hematoxylin has in our hands proven dependable for routine staining of serial 2\u2010m\u0308m glycol methacrylate sections used in mapping the distributions of these cells along the airway. In lungs of mice, hamsters, kittens, and fetal rabbits, typical small\u2010granule cells stain weakly or not at all with lead hematoxylin alone, hence are easily overlooked. PAS adds to the cytoplasm a diffuse magenta coloration; and because it is diastase\u2010resistant, less brilliant than that of mucus but more so than bronchiolar cell secretions, and finer textured than lysosomal staining of other cells present, the effect is to highlight small\u2010granule cells whether solitary or in clusters. Additional PAS staining of basement membranes and lead hematoxylin staining of cilia enhance the combined stain's resolving power. In thyroid gland, parafollicular cells stand out boldly against follicular elements; in small intestine, hematoxylin\u2010positive endocrine cells are well differentiated from absorptive, mucous, and Paneth cells that surround them. Using a complementary monoamine fluorescence technique on plastic sections of lungs from control and 5\u2010hydroxytryptophan\u2010pretreated animals prior to staining, we can show that fluorescent epithelial cells are identical with those stained by PAS\u2010lead hema\u2010toxylin.<\/jats:p>","DOI":"10.1002\/ar.1091920205","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T06:58:20Z","timestamp":1109401100000},"page":"245-259","source":"Crossref","is-referenced-by-count":53,"title":["PAS\u2010lead hematoxylin as a stain for small\u2010granule endocrine cell populations in the lungs, other pharyngeal derivatives and the gut"],"prefix":"10.1002","volume":"192","author":[{"given":"Sergei P.","family":"Sorokin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"Richard F.","family":"Hoyt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,1,26]]},"reference":[{"key":"e_1_2_1_2_1","volume-title":"Principles of Biological Microtech\u2010nique: A Study of Fixation and Dyeing","author":"Baker J. 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Invest."},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/158202a0"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1177\/17.5.303"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/2211210a0"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.3109\/10520296709115020"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00268705"},{"key":"e_1_2_1_21_1","first-page":"13","article-title":"Revised Wiesbaden classification of gut endocrine cells","volume":"5","author":"Solcia E.","year":"1973","journal-title":"Rendic. Gastroenterol."},{"key":"e_1_2_1_22_1","first-page":"3","volume-title":"Morphology of Respiratory Carcinogenesis: AEC Symposium Series 21 (CONF\u20107000501, National Technical Information Service","author":"Sorokin S. P.","year":"1970"},{"key":"e_1_2_1_23_1","first-page":"765","volume-title":"Histology","author":"Weiss L.","year":"1977"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(59)90023-5"}],"container-title":["The Anatomical Record"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Far.1091920205","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ar.1091920205","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,12]],"date-time":"2023-11-12T05:24:12Z","timestamp":1699766652000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ar.1091920205"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1978,10]]},"references-count":23,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1978,10]]}},"alternative-id":["10.1002\/ar.1091920205"],"URL":"https:\/\/doi.org\/10.1002\/ar.1091920205","archive":["Portico"],"relation":{},"ISSN":["0003-276X","1097-0185"],"issn-type":[{"value":"0003-276X","type":"print"},{"value":"1097-0185","type":"electronic"}],"subject":[],"published":{"date-parts":[[1978,10]]}}}