{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:18:41Z","timestamp":1762100321644},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,8]],"date-time":"2005-02-08T00:00:00Z","timestamp":1107820800000},"content-version":"vor","delay-in-days":8684,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Anat. Rec."],"published-print":{"date-parts":[[1981,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Odontoblasts are cells with single cytoplasmic processes that grow longer as more dentin is elaborated. Ameloblasts also have single processes and it has been postulated that they too grow longer as more enamel is made. Support for this hypothesis was obtained using rat incisors to investigate the behavior of substances labeled with <jats:sup>3<\/jats:sup>H\u2010proline and <jats:sup>3<\/jats:sup>H\u2010fucose. A comparison was made between odontoblasts, which have processes known to grow and remain within the dentin, and the ameloblasts whose Tomes' processes are hypothesized to grow and leave remnants in the completed enamel. With <jats:sup>3<\/jats:sup>H\u2010proline, the odontoblast bodies are labeled at the early time intervals. They synthesize and secrete a layer of intensely labeled predentin, which by 1 and 2 days is converted to mineralized dentin. Matrix deposited after the main pulse is weakly labeled. Odontoblast processes are never labeled in dentin formed prior to injection. With <jats:sup>3<\/jats:sup>H\u2010fucose, the cell bodies are labeled at the early intervals and the newly formed glycoproteins are deposited into the predentin. Almost immediately, these are progressively added to the dentin at the calcification front. With time a gradient of labeling extends from the unlabeled dentin toward the odontoblast bodies. Unlike the behavior of labeled proteins, by 1 and 2 days labeled glycoproteins appear along the entire length of the odontoblast processes. In the enamel, no Tomes' processes are present during maturation. With <jats:sup>3<\/jats:sup>H\u2010proline, reactions are adjacent to the cells and diffuse toward, but do not reach the dentino\u2010enamel junction by 1 and 2 days. With <jats:sup>3<\/jats:sup>H\u2010fucose, reactions appear over the enamel near the cells. By 1 and 2 days no diffusive pattern is seen, but grains are concentrated near the dentino\u2010enamel junction, in a region containing holes known to be the beginning of Tomes' processes. Since odontoblast glycoproteins migrate along odontoblast processes, it was postulated that cytoplasmic remnants were present in enamel along which ameloblast glycoproteins could also migrate to reach the holes at the dentino\u2010enamel junction.<\/jats:p>","DOI":"10.1002\/ar.1092000102","type":"journal-article","created":{"date-parts":[[2005,2,27]],"date-time":"2005-02-27T00:05:50Z","timestamp":1109462750000},"page":"1-10","source":"Crossref","is-referenced-by-count":39,"title":["The behavior of substances labeled with <sup>3<\/sup>H\u2010proline and <sup>3<\/sup>H\u2010fucose in the cellular processes of odontoblasts and ameloblasts"],"prefix":"10.1002","volume":"200","author":[{"given":"H.","family":"Warshawsky","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Josephsen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,8]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/003591576706000965"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(59)90008-4"},{"key":"e_1_2_1_4_1","first-page":"45","article-title":"Ameloblast modulation in the maturation zone of the rat incisor enamel organ. A light and electron microscopic study","volume":"124","author":"Josephsen K.","year":"1977","journal-title":"J. Anat."},{"key":"e_1_2_1_5_1","unstructured":"Josephsen K. J.Theilade andO.Fejerskov(1974)Perfusion fixation of rat incisor enamel organ. Effect of buffer concentration. Scandinavian Division of IADR General Meeting Abstract 91."},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1091950403"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/10.3.269"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345790580024901"},{"key":"e_1_2_1_9_1","first-page":"685","article-title":"An unusual structure associated with the onset of prism formation in rat incisor enamel. An electron microscopic study","volume":"74","author":"Nylen M. U.","year":"1970","journal-title":"Tandl\u00e6gebladet (Danish Dental Journal)"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(74)90093-4"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001450206"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1091690307"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001520203"},{"key":"e_1_2_1_14_1","first-page":"105","article-title":"Radioautographic studies of amelogenesis","volume":"7","author":"Warshawsky H.","year":"1979","journal-title":"J. Biol. Buccale"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1177\/15.9.542"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1091790403"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1091880203"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001170206"},{"key":"e_1_2_1_19_1","first-page":"121","volume-title":"Biochemistry and Physiology of Bone","author":"Weinstock A.","year":"1972"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.51.1.26"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02010136"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.60.1.92"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(63)90068-2"}],"container-title":["The Anatomical Record"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Far.1092000102","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ar.1092000102","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,12]],"date-time":"2023-11-12T05:49:41Z","timestamp":1699768181000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ar.1092000102"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1981,5]]},"references-count":22,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1981,5]]}},"alternative-id":["10.1002\/ar.1092000102"],"URL":"https:\/\/doi.org\/10.1002\/ar.1092000102","archive":["Portico"],"relation":{},"ISSN":["0003-276X","1097-0185"],"issn-type":[{"value":"0003-276X","type":"print"},{"value":"1097-0185","type":"electronic"}],"subject":[],"published":{"date-parts":[[1981,5]]}}}