{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T06:50:11Z","timestamp":1760424611152},"reference-count":17,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,1,26]],"date-time":"2005-01-26T00:00:00Z","timestamp":1106697600000},"content-version":"vor","delay-in-days":6784,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Anat. Rec."],"published-print":{"date-parts":[[1986,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A scanning electron microscopy, histological, and immunochemical investigation examined the effects of the mutant gene (e) upon hypothalamic development in the Mexican axolotl. The adult eyeless mutant is sterile. Previous studies indicated that this reproductive defect was due to the mutation's effect upon the hypothalamus.<\/jats:p><jats:p>The present study demonstrated the pleiotropic effects of the eyeless gene upon development of the hypothalamus. Scanning electron microscopy studies looked at the early ontogeny of the hypothalamohypophyseal system. The major morphological difference observed in the hypothalamus of normals compared to eyeless mutants was the reduced nature or complete lack of a preoptic recess in eyeless mutants. Early embryonic tissue movements also differed when normal siblings were compared to eyeless mutant axolotls. Histological examination looking for paraldehyde\u2010fuchsin\u2010positive secretory neurons revealed a paired nucleus preopticus in both normals and eyeless mutants, but this region lacked the emanating paraldehyde\u2010fuchsin\u2010positive fiber tracts in eyeless mutants. The neurohypophysis of the eyeless mutants was atrophied and contained far less paraldehyde\u2010fuchsin\u2010positive material when compared to normal axolotls.<\/jats:p><jats:p>Immunochemical studies were done to look at the distribution of immunoreactive luteinizing\u2010hormone\u2010releasing hormone (ir\u2010LHRH) in brains of eyed and eyeless mutant axolotls of different stages. This study detected deficiencies in ir\u2010LHRH in the anterior hypothalamus of eyeless mutants.<\/jats:p><jats:p>In general in the eyeless mutant axolotl, the observed anterior hypothalamic deficiencies are comparable to those observed in anurans which have had their optic vesicles removed. These observations suggest a possible utility of the eyeless mutant axolotl for studies concerned with endocrine development in the absence of hypothalamic modulation.<\/jats:p>","DOI":"10.1002\/ar.1092150314","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T23:02:40Z","timestamp":1109458960000},"page":"317-327","source":"Crossref","is-referenced-by-count":5,"title":["A scanning electron microscopy and histological study on the effects of the mutant eyeless (e\/e) gene upon the hypothalamus in the Mexican axolotl <i>Ambystoma mexicanum<\/i> Shaw"],"prefix":"10.1002","volume":"215","author":[{"given":"Gerald W.","family":"Eagleson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George M.","family":"Malacinski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(80)90375-9"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-66316-1_33"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(82)90225-1"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1093\/icb\/18.2.267","article-title":"A review of the eyeless mutant in the Mexican axolotl","volume":"181","author":"Epp L. G.","year":"1978","journal-title":"Am. Zool."},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4886(76)90239-9"},{"key":"e_1_2_1_7_1","first-page":"206A","article-title":"A recently discovered mutant \u201ceyeless\u201d in the Mexican axolotl, Ambystoma mexicanum","volume":"163","author":"Humphrey R. R.","year":"1969","journal-title":"Anat. Rec."},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402070303"},{"key":"e_1_2_1_9_1","first-page":"1","volume-title":"Basic Structure and Evolution of Vertebrates","author":"Jarvik E.","year":"1981"},{"key":"e_1_2_1_10_1","volume-title":"The Central Nervous System of Vertebrates","author":"Kuhlenbeck H.","year":"1973"},{"key":"e_1_2_1_11_1","first-page":"325","article-title":"Some morphogenetic features of the adenohypophyseal primordium of early Xenopus laevis tadpoles","volume":"168","author":"Nyholm N. E. I.","year":"1977","journal-title":"Cell Tissue Res."},{"key":"e_1_2_1_12_1","first-page":"22","volume-title":"A Student's Guide to Laboratory Experiments in General and Comparative Endocrinology","author":"Peter R. E.","year":"1970"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(75)90343-7"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4886(77)90018-8"},{"key":"e_1_2_1_15_1","unstructured":"Schwers J.(1984)Distinguishing pituitary thyrotropes from gonado\u2010tropes in the axolotl using antisera to \u03b2\u2010LH. BSc thesis: Loras College Dubuque Iowa."},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00315713"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(73)90344-8"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1679\/aohc1950.43.157"}],"container-title":["The Anatomical Record"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Far.1092150314","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ar.1092150314","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T22:51:20Z","timestamp":1697842280000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ar.1092150314"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1986,7]]},"references-count":17,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1986,7]]}},"alternative-id":["10.1002\/ar.1092150314"],"URL":"https:\/\/doi.org\/10.1002\/ar.1092150314","archive":["Portico"],"relation":{},"ISSN":["0003-276X","1097-0185"],"issn-type":[{"value":"0003-276X","type":"print"},{"value":"1097-0185","type":"electronic"}],"subject":[],"published":{"date-parts":[[1986,7]]}}}