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The latter were intermediate in morphology between GCT cells and striated duct cells. Both large and small granules were immunostained in GCT cells; however, considerable variability in immunostaining intensity was observed in both sizes of granules but especially in the small granules of transition cells. Rough endoplasmic reticulum (RER) in both cell types exhibited NGF immunoreactivity. No Golgi\u2010associated immunostaining was observed.<\/jats:p><jats:p>Following \u03b1\u2010adrenergic stimulation with phenylephrine, NGF\u2010containing granules were sharply reduced because of extensive degranulation. Pools of immunostained secretory material suggested intracellular fusion of NGF\u2010containing granules. Immunostaining was also observed on membrane fragments found within large vacuoles in GCT cells. Evidence of NGF secretion after \u03b2\u2010adrenergic or cholinergic stimulation was less dramatic. In isoproterenol\u2010stimulated GCT cells there was evidence of fusion of small, apical, NGF\u2010stained granules. These cells also possessed heavily immunostained apical membrane blebs. Pilocarpine\u2010stimulated cells exhibited pleomorphic immunostained apical granules but less apical membrane immunostaining. Abundant basal lysosomes appearing in GCT cells after pilocarpine stimulation did not stain for NGF.<\/jats:p>","DOI":"10.1002\/ar.1092190210","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T22:47:38Z","timestamp":1109458058000},"page":"171-179","source":"Crossref","is-referenced-by-count":9,"title":["Electron microscopic immunostaining of nerve growth factor: Secretagogue stimulated submandibular glands"],"prefix":"10.1002","volume":"219","author":[{"given":"Debra J.","family":"Hazen\u2010Martin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. A. 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