{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T17:05:36Z","timestamp":1760547936570},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,8]],"date-time":"2005-02-08T00:00:00Z","timestamp":1107820800000},"content-version":"vor","delay-in-days":6248,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Anat. Rec."],"published-print":{"date-parts":[[1988,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To examine whether either of the two known active vitamin D metabolites 1,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub> or 24,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub> could reverse the mineralization defect induced by 1\u2010hydroxyethylidene\u20101,1\u2010bis phosphonate (EHDP), a model of EHDP\u2010induced rickets was used. Rats at the age of 31 days were injected for 10 consecutive days with EHDP (10 mg\/kg). Other littermates were treated with a combination of EHDP and either 1,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub> or 24,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub> or were treated following 10 days of EHDP, with either of the vitamin D metabolites for an additional 72 hr. Samples of cartilage fluid (Cfl) and of blood were removed prior to sacrifice for biochemical studies of some parameters of calcification. These parameters were correlated with the results of light and electron microscope studies of growth plate cartilage and bone. EHDP\u2010treated rats revealed signs of typical rickets, manifested by widened growth plates and impaired bone mineralization. Transmission electron microscope (TEM) examination revealed matrix vesicles distributed throughout the growth plate; however, there appeared to be an arrest of the spread of the crystals at the provisional zone of calcification. Treatment with either 1,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub> or 24,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub> failed to reverse the rachitic condition of the animals. Serum calcium blood levels were elevated in the 1,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub> and EHDP\u2010treated group. 1,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub> and 24,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub> further increased the already elevated serum alkaline phosphatase levels observed in EHDP rats, although the increase observed with 1,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub> was not statistically significant. In contrast to this, 24,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub> lowered the Cfl levels of alkaline phosphatase to the normal range despite the absence of apparent morpholoogical healing of the EHDP rachitic condition. It therefore appears that in our animal model pharmacological doses of vitamin D metabolites do not reverse the EHDP\u2010induced mineralization defect.<\/jats:p>","DOI":"10.1002\/ar.1092200104","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T22:33:09Z","timestamp":1109457189000},"page":"22-30","source":"Crossref","is-referenced-by-count":5,"title":["EHDP\u2010induced rachitic syndrome in rats is not reversed by vitamin D metabolites"],"prefix":"10.1002","volume":"220","author":[{"given":"Isaac","family":"Atkin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asher","family":"Ornoy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julio C.","family":"Pita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ofelia E.","family":"Muniz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agueda","family":"Agundez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Greg","family":"Castiglione","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David S.","family":"Howell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,8]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/8756-3282(85)90315-1"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(74)90077-0"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02441220"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1152\/ajplegacy.1973.224.5.1011","article-title":"Intestinal calcium absorption, Ca\u2010binding protein, and Ca\u2010ATPase in diphosphonate\u2010treated rats","volume":"224","author":"Bonjour J. P.","year":"1972","journal-title":"Am. J. Physiol."},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2362.1973.tb00328.x"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1152\/ajplegacy.1975.229.2.402","article-title":"Action of 1,25\u2010dihydroxyvitamin D3 and diphosphonate on calcium metabolism in rats","volume":"229","author":"Bonjour J. P.","year":"1975","journal-title":"Am. J. Physiol."},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"1899","DOI":"10.1093\/jn\/108.12.1899","article-title":"Evidence for the promotion of bone mineralization by 1\u03b1,25\u2010dihydroxycholecalciferol in the rat unrelated to the correction of deficiencies in serum calcium and phosphorous","volume":"108","author":"Boris A.","year":"1978","journal-title":"J. Nutrition"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0221-8747(79)90017-1"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0221-8747(81)90044-8"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02058658"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1042\/cs0430031"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1042\/cs0440335"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.3109\/03009737709179080"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI105801"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02408604"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003086-197107000-00021"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02547384"},{"key":"e_1_2_1_19_1","first-page":"17","article-title":"Light microscopic and ultrastructural observations on the short\u2010term effects of ethylene\u20101\u2010hydroxy\u20101,\u2010diphosphonate (EHDP) on rat tibia epiphysis","volume":"84","author":"Larsson A.","year":"1976","journal-title":"Acta Pathol. Microbiol. Scand."},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02546241"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/276517a0"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(78)90015-5"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02408548"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003086-197003000-00008"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02547218"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02547219"}],"container-title":["The Anatomical Record"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Far.1092200104","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ar.1092200104","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T18:07:48Z","timestamp":1697911668000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ar.1092200104"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,1]]},"references-count":25,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1988,1]]}},"alternative-id":["10.1002\/ar.1092200104"],"URL":"https:\/\/doi.org\/10.1002\/ar.1092200104","archive":["Portico"],"relation":{},"ISSN":["0003-276X","1097-0185"],"issn-type":[{"value":"0003-276X","type":"print"},{"value":"1097-0185","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,1]]}}}