{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,10]],"date-time":"2025-09-10T21:47:05Z","timestamp":1757540825400},"reference-count":50,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,1,26]],"date-time":"2005-01-26T00:00:00Z","timestamp":1106697600000},"content-version":"vor","delay-in-days":6204,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Anat. Rec."],"published-print":{"date-parts":[[1988,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Acute, intense sources of \u201cpsychogenic\u201d stress clearly modify the structure and function of the hypophysis, and there are concomitant changes in many peripheral physiological systems. Less dramatic sources of stress yield more equivocal results. An experiment is reported in which nuclear morphology of adenohypophyseal cells from 49 male rats exposed to a chronic, low\u2010intensity stressor was examined both by conventional histological and computer\u2010assisted\u2010image\u2010processing methods. The hypothesis tested was that an unequivocal pattern of morphological changes in the nucleus and nuclear chromatin would be revealed by image processing. Rats were killed after living for a year in a relatively low\u2010stress environment, \u201ccrowded\u201d in groups of five animals per cage. The control condition was a minimal stress environment of two rats per cage. Results suggested few signs of pathology from peripheral measures of hypophyseal activity, and direct light microscopic examination of the gland revealed no differences between the two groups. Analysis of computer\u2010enhanced images of the pars distalis nuclei from the adenohypophysis, on the other hand, generated findings that were statistically and biologically significant. Nuclear size increased in the stress condition, the number of chromatin and area occupied by the particles increased, and the position of chromatin shifted toward the periphery of the nucleus. Perhaps more important, optical density analysis indicated that chromatin was less tightly packed in the experimental animals. Implications are that chronic, low\u2010intensity stress modulates nuclear structural changes from a dormant to an active state that portend changes in the peripheral systems influenced by the hypophysis.<\/jats:p>","DOI":"10.1002\/ar.1092200203","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T22:34:17Z","timestamp":1109457257000},"page":"125-131","source":"Crossref","is-referenced-by-count":5,"title":["Structural changes in nuclear chromatin in rat pituitary after chronic stress of low intensity"],"prefix":"10.1002","volume":"220","author":[{"given":"Dymitr","family":"Komitowski","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuhei","family":"Muto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00fcrgen","family":"Weiss","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bertold","family":"Schmitt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George T.","family":"Taylor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,1,26]]},"reference":[{"key":"e_1_2_1_2_1","volume-title":"Molecular Biology of the Cell","author":"Alberts B.","year":"1983"},{"key":"e_1_2_1_3_1","first-page":"235","volume-title":"The Pituitary","author":"Allen M. 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